Subcutaneous immunization with a novel immunogenic candidate (urease) confers protection against Brucella abortus and Brucella melitensis infections

被引：18

作者：

Abkar, Morteza ^{[1
]}

Amani, Jafar ^{[2
]}

Lotfi, Abbas Sahebghadam ^{[3
]}

Brujeni, Gholamreza Nikbakht ^{[4
]}

Alamian, Saeed ^{[5
]}

Kamali, Mehdi ^{[6
]}

机构：

[1] Tarbiat Modares Univ, Dept Mol Genet, Fac Basic Sci, Tehran, Iran

[2] Baqiyatallah Univ Med Sci, Appl Microbiol Res Ctr, Tehran, Iran

[3] Tarbiat Modares Univ, Dept Clin Biochem, Fac Med, Tehran, Iran

[4] Univ Tehran, Fac Vet Med, Dept Microbiol & Immunol, Tehran, Iran

[5] Razi Vaccine & Serum Res Inst, Brucellosis Dept, Karaj, Iran

[6] Baqiyatallah Univ Med Sci, Nanobiotechnol Res Ctr, Tehran, Iran

来源：

APMIS | 2015年 / 123卷 / 08期

基金：

美国国家科学基金会;

关键词：

Brucellosis; urease; subcutaneous; intraperitoneal; subunit vaccine; IMMUNE-RESPONSE; VACCINE ADJUVANT; MICE; TRAFFICKING;

D O I：

10.1111/apm.12400

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Brucellosis is a world prevalent endemic illness that is transmitted from domestic animals to humans. Brucella spp. exploits urease for survival in the harsh conditions of stomach during the gastrointestinal infection. In this study, we examined the immune response and the protection elicited by using recombinant Brucella urease (rUrease) vaccination in BALB/c mice. The urease gene was cloned in pET28a and the resulting recombinant protein was employed as subunit vaccine. Recombinant protein was administered subcutaneously and intraperitoneally. Dosage reduction was observed with subcutaneous (SC) vaccination when compared with intraperitoneal (IP) vaccination. rUrease induced mixed Th1-Th2 immune responses with high titers of specific IgG1 and IgG2a. In lymphocyte proliferation assay, splenocytes from IP and SC-vaccinated mice displayed a strong recall proliferative response with high amounts of IL-4, IL-12 and IFN- production. Vaccinated mice were challenged with virulent Brucella melitensis, B.abortus and B.suis. The SC vaccination route exhibited a higher degree of protection than IP vaccination (p value0.05). Altogether, our results indicated that rUrease could be a useful antigen candidate for the development of subunit vaccines against brucellosis.

引用

页码：667 / 675

页数：9

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