Bisphenol a reduces fertilizing ability and motility by compromising mitochondrial function of sperm

被引：26

作者：

Singh, Ram P. ^{[1
,2
]}

Shafeeque, Chathathayil M. ^{[1
]}

Sharma, Sanjeev K. ^{[3
]}

Pandey, Nitin K. ^{[3
]}

Singh, Renu ^{[5
]}

Mohan, Jag ^{[3
]}

Kolluri, Gautham ^{[3
]}

Saxena, Meeta ^{[4
]}

Sharma, Bhaskar ^{[4
]}

Sastry, Kochiganti V. H. ^{[3
]}

Kataria, Jag M. ^{[3
]}

Azeez, Parappurath A. ^{[1
]}

机构：

[1] Salim Ali Ctr Ornithol & Nat Hist, Avian Physiol & Genet Div, Coimbatore, Tamil Nadu, India

[2] Smithsonian Conservat Biol Inst, Front Royal, VA USA

[3] Cent Avian Res Inst, Div Physiol & Reprod, Izatnagar, Uttar Pradesh, India

[4] Indian Vet Res Inst, Div Biochem, Izatnagar 243122, Uttar Pradesh, India

[5] Indian Vet Res Inst, Div Physiol & Climatol, Izatnagar 243122, Uttar Pradesh, India

来源：

ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY | 2015年 / 34卷 / 07期

关键词：

Sperm viability; Flow cytometer; Mitochondrial membrane potential; Fertility; SPERMATOZOA; EXPOSURE;

D O I：

10.1002/etc.2957

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

Bisphenol A (BPA) acts as an endocrine disruptor, affects animal reproductive success in vivo and affects sperm functions in vitro at environmentally relevant concentrations, leading to reduction in sperm motility and fertilizing ability in fish. The effect of in vitro BPA on avian sperm functions has not been explored. The present study examined the effect of environmentally relevant concentrations of BPA (0mM, 0.18mM, 0.37mM, and 0.74mM) on sperm functions in chicken in vitro. Sperm were exposed to concentrations of BPA for 30 min and analyzed for motility, fertilizing ability, live sperm percentage, and mitochondrial membrane potential (m). Results showed that BPA at a concentration of 0.74 mM significantly decreased motility, fertilizing ability, live sperm count percentage, and sperm m. Sperm motility was positively correlated with fertility (r =0.73, p 0.01), live sperm percentage (r=0.64, p0.01), and high m (r=0.44, p0.01). A dose-dependent and time-dependent effect of BPA was observed on sperm motility at all BPA concentrations. However, sperm's fertilizing ability was unaffected in low BPA concentration (0.18mM and 0.37mM). A significantly higher percentage of moribund sperm was observed at 0.37mM and 0.74mM BPA compared with at 0.18mM BPA, in the negative control, and in the vehicle control. The present study confirms that environmentally relevant concentrations of BPA are capable of compromising sperm functions, leading to reduction in fertilizing ability of chicken sperm. Environ Toxicol Chem 2015;34:1617-1622. (c) 2015 SETAC

引用

页码：1617 / 1622

页数：6

共 19 条

[1] GENERATION OF REACTIVE OXYGEN SPECIES, LIPID-PEROXIDATION, AND HUMAN-SPERM FUNCTION [J].