Performing 2D-1D-2D Mass Spectrometry Imaging Using Strings

The mass spectrometry imaging (MSI) technique is widely used in several fields due to its ability to provide spatial information of samples. However, for existing MSI methods, the sample is typically placed on a two-dimensional (2D) platform and is scanned back and forth. As a result, the platform size limits the imaging size. This paper proposes a new MSI method that involves the initial imprinting of chemicals on a two-dimensional string plane area. The string plane was then unraveled to a onedimensional (1D) string, and the chemicals imprinted on it were ionized using a lab-made ion source. Finally, a 2D MSI image was reconstructed through data processing (2D-1D-2D mass imaging). Compared with traditional MSI methods, the imaging size is no longer limited by the platform size, making it possible to perform the MSI of large samples. As proof of concept, this method was used to image an intact seedling of Broussonetia papyrifera. As a result, clear and overall MS images were obtained, demonstrating the ability of this method to analyze large samples.