The LAH4 family of amphipathic peptides exhibits pronounced antimicrobial, cell penetrating and nucleic acid transfection activities. Furthermore, variants were designed with potent lentiviral transduction enhancement. When viewed along a helical wheel the four histidines are arranged to form an amphipathic structure. In order to optimize some of these biological activities the number of leucine and alanine residues exposed to the hydrophilic surface was systematically varied which resulted in the design of vectofusin a peptide with strong lentiviral transduction enhancement activities. Here the series of peptides with varying numbers of alanine or leucine residues, respectively, framed by the histidines was tested for their calcein release activity. Interestingly, the membrane pore formation and DNA transfection activities show a clear correlation with the hydrophilic angle. In contrast the membrane partitioning and the propensity to adopt helical conformations was hardly affected as long as the hydrophilic angle did not exceed a limiting value of 150 degrees.
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US Naval Res Lab, Ctr Bio Mol Sci & Engn Code 6900, Washington, DC 20375 USAUS Naval Res Lab, Ctr Bio Mol Sci & Engn Code 6900, Washington, DC 20375 USA
Gemmill, Kelly Boeneman
Muttenthaler, Markus
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Scripps Res Inst, Dept Chem, La Jolla, CA 92037 USA
Scripps Res Inst, Dept Cell Biol, La Jolla, CA 92037 USAUS Naval Res Lab, Ctr Bio Mol Sci & Engn Code 6900, Washington, DC 20375 USA
Muttenthaler, Markus
Delehanty, James B.
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US Naval Res Lab, Ctr Bio Mol Sci & Engn Code 6900, Washington, DC 20375 USAUS Naval Res Lab, Ctr Bio Mol Sci & Engn Code 6900, Washington, DC 20375 USA
Delehanty, James B.
Stewart, Michael H.
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US Naval Res Lab, Div Opt Sci Code 5600, Washington, DC 20375 USAUS Naval Res Lab, Ctr Bio Mol Sci & Engn Code 6900, Washington, DC 20375 USA
Stewart, Michael H.
Susumu, Kimihiro
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US Naval Res Lab, Div Opt Sci Code 5600, Washington, DC 20375 USA
Sotera Def Solut, Annapolis Jct, MD 20701 USAUS Naval Res Lab, Ctr Bio Mol Sci & Engn Code 6900, Washington, DC 20375 USA
Susumu, Kimihiro
Dawson, Philip E.
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Scripps Res Inst, Dept Chem, La Jolla, CA 92037 USA
Scripps Res Inst, Dept Cell Biol, La Jolla, CA 92037 USAUS Naval Res Lab, Ctr Bio Mol Sci & Engn Code 6900, Washington, DC 20375 USA
Dawson, Philip E.
Medintz, Igor L.
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US Naval Res Lab, Ctr Bio Mol Sci & Engn Code 6900, Washington, DC 20375 USAUS Naval Res Lab, Ctr Bio Mol Sci & Engn Code 6900, Washington, DC 20375 USA
机构:
US Naval Res Lab, Ctr Bio Mol Sci & Engn Code 6900, Washington, DC 20375 USAUS Naval Res Lab, Ctr Bio Mol Sci & Engn Code 6900, Washington, DC 20375 USA
Gemmill, Kelly Boeneman
Muttenthaler, Markus
论文数: 0引用数: 0
h-index: 0
机构:
Scripps Res Inst, Dept Chem, La Jolla, CA 92037 USA
Scripps Res Inst, Dept Cell Biol, La Jolla, CA 92037 USAUS Naval Res Lab, Ctr Bio Mol Sci & Engn Code 6900, Washington, DC 20375 USA
Muttenthaler, Markus
Delehanty, James B.
论文数: 0引用数: 0
h-index: 0
机构:
US Naval Res Lab, Ctr Bio Mol Sci & Engn Code 6900, Washington, DC 20375 USAUS Naval Res Lab, Ctr Bio Mol Sci & Engn Code 6900, Washington, DC 20375 USA
Delehanty, James B.
Stewart, Michael H.
论文数: 0引用数: 0
h-index: 0
机构:
US Naval Res Lab, Div Opt Sci Code 5600, Washington, DC 20375 USAUS Naval Res Lab, Ctr Bio Mol Sci & Engn Code 6900, Washington, DC 20375 USA
Stewart, Michael H.
Susumu, Kimihiro
论文数: 0引用数: 0
h-index: 0
机构:
US Naval Res Lab, Div Opt Sci Code 5600, Washington, DC 20375 USA
Sotera Def Solut, Annapolis Jct, MD 20701 USAUS Naval Res Lab, Ctr Bio Mol Sci & Engn Code 6900, Washington, DC 20375 USA
Susumu, Kimihiro
Dawson, Philip E.
论文数: 0引用数: 0
h-index: 0
机构:
Scripps Res Inst, Dept Chem, La Jolla, CA 92037 USA
Scripps Res Inst, Dept Cell Biol, La Jolla, CA 92037 USAUS Naval Res Lab, Ctr Bio Mol Sci & Engn Code 6900, Washington, DC 20375 USA
Dawson, Philip E.
Medintz, Igor L.
论文数: 0引用数: 0
h-index: 0
机构:
US Naval Res Lab, Ctr Bio Mol Sci & Engn Code 6900, Washington, DC 20375 USAUS Naval Res Lab, Ctr Bio Mol Sci & Engn Code 6900, Washington, DC 20375 USA