Development of a microfluidic device using nanoparticle-based bio-barcodes for ultrasenstitive detection of proteins

被引：0

作者：

Goluch, ED ^{[1
]}

Nam, JM ^{[1
]}

Chiesl, TN ^{[1
]}

Shaikh, KA ^{[1
]}

Ryu, KS ^{[1
]}

Barron, AE ^{[1
]}

Mirkin, CA ^{[1
]}

Liu, C ^{[1
]}

机构：

[1] Univ Illinois, Micro Acturators Sensors & Syst Grp, Urbana, IL 61801 USA

来源：

Micro Total Analysis Systems 2004, Vol 1 | 2005年 / 296期

关键词：

protein detection; bio-bar-code; polyduramide; nanoparticles;

D O I：

暂无

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We present a microfluidic system that allows for ultra-sensitive protein detection. The system integrates the bio-bar-code amplification (BCA) [1] approach with silver staining on a single microfluidic device to detect Prostate Specific Antigen in concentrations as low as 500 attomolar (300 proteins in the entire sample), using several orders of magnitude less sample volume than necessary for the bench-top assay.

引用

页码：342 / 344

页数：3

共 3 条

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SCIENCE, 2003, 301 (5641) :1884-1886

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Unger, MA ;

Chou, HP ;

Thorsen, T ;

Scherer, A ;

Quake, SR .

SCIENCE, 2000, 288 (5463) :113-116

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