Effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin on spontaneous movement of human neuroblastoma cells

被引:11
作者
Luo, Yali [1 ,2 ]
Xu, Tuan [1 ,2 ]
Xie, Heidi Qunhui [1 ,2 ]
Guo, Zhiling [1 ,3 ]
Zhang, Wanglong [1 ,2 ]
Chen, Yangsheng [1 ,2 ]
Sha, Rui [1 ,2 ]
Liu, Yiyun [1 ,2 ]
Ma, Yongchao [1 ,2 ]
Xu, Li [1 ,2 ]
Zhao, Bin [1 ,2 ,4 ]
机构
[1] Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Chem & Ecotoxicol, Beijing 100085, Peoples R China
[2] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
[3] Univ Birmingham, Sch Geog Earth & Environm Sci, Birmingham B15 2TT, W Midlands, England
[4] Univ Chinese Acad Sci, Inst Environm & Hlth, Hangzhou Inst Adv Study, Hangzhou 310024, Peoples R China
基金
中国国家自然科学基金;
关键词
Dioxin; Neuroblastoma; Spontaneous movement; Aryl hydrocarbon receptor; Cell adhesion; HYDROCARBON RECEPTOR AHR; MOTILITY FACTOR; DIOXIN; EXPRESSION; MIGRATION; MODEL; PROLIFERATION; POPULATION; MORTALITY; ADHESION;
D O I
10.1016/j.scitotenv.2020.136805
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
Aryl hydrocarbon receptor (AhR) plays important roles in the interferences of dioxin exposure with the occurrence and development of tumors. Neuroblastoma is a kind of malignant tumor with high mortality and its occurrence is getting higher in dioxin exposed populations. However, there is still a lack of direct evidence of influences of dioxin on neuroblastoma cell migration. SK-N-SH is a human neuroblastoma cell line which has been used to reveal 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)-induced dysregulation of certain promigratory gene. Thus, in this study, we employed SK-N-SH cells to investigate the effects of TCDD on the spontaneous movement of neuroblastoma cells, which is a short-range cell migratory behavior related to done formation and tumor metastasis in vitro. Using unlabeled live cell imaging and high content analysis, we characterized the spontaneous movement under a full-nutrient condition in SK-N-SH cells. We found that the spontaneous movement of SK-N-SH cells was inhibited after 36- or 48-h treatment with TCDD at relative low concentrations (10(-10) or 2 x 10(-10) M). The TCDD-treated cells were unable to move as freely as that of control cells, resulting in less diffusive trajectories and a decreased displacement of the movement. In line with this cellular effect, the expression of pro-adhesive genes was significantly induced in time- and concentration-dependent manners after TCDD treatment. In addition, with the presence of AhR antagonist, CH223191, the effects of TCDD on the gene expression and the spontaneous cell movement were effectively reversed. Thus, we proposed that AhR-mediated upregulation of pro-adhesive genes might be involved in the inhibitory effects of dioxin on the spontaneous movement of neuroblastoma cells. To our knowledge, this is the first piece of direct evidence about the influence of dioxin on neuroblastoma cell motility. (C) 2020 Published by Elsevier B.V.
引用
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页数:10
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