Modulation of DNA polymerase function for DNA diagnostics

被引：0

作者：

Marx, A ^{[1
]}

机构：

[1] Univ Konstanz, Fachbereich Chem, Lehrstuhl Organ Chem & Zellulare Chem, D-78457 Constance, Germany

来源：

Chemistry of Nucleic Acid Components | 2005年 / 7卷

关键词：

D O I：

暂无

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We reported on two approaches to modulate and increase single nucleotide discrimination of DNA polymerase for applications in DNA diagnostics using PCR. We show that certain chemical modifications in primer probes are able to increase the discrimination in favor of extension of canonical primer probes versus mismatched primer probes. In a second approach to modulate DNA polymerases in this respect we employed directed combinatorial enzyme design to identify enzymes with increased mismatch extension fidelity.

引用

页码：123 / 126

页数：4

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