Optimization of mannanase production by Bacillus sp HDYM-05 through factorial method and response surface methodology

被引：4

作者：

Zhao Dan ^{[1
,2
,3
]}

Ping Wenxiang ^{[1
,2
,3
]}

Song Gang ^{[1
,2
,3
]}

Ling Hongzhi ^{[1
,2
,3
]}

Luo Xue ^{[1
]}

Ge Jingping ^{[1
,2
,3
]}

机构：

[1] Heilongjiang Univ, Microbiol Lab, Coll Life Sci, Harbin 150080, Peoples R China

[2] Heilongjiang Univ, Engn Res Ctr Agr Microbiol Technol, Minist Educ, Harbin 150080, Peoples R China

[3] Heilongjiang Univ, Coll Heilongjiang Prov, Key Lab Mol Biol, Harbin 150080, Peoples R China

来源：

AFRICAN JOURNAL OF MICROBIOLOGY RESEARCH | 2012年 / 6卷 / 01期

基金：

中国国家自然科学基金;

关键词：

Mannanase; Bacillus cereus; optimization; factorial method; response surface methodology; ALKALINE PROTEASE PRODUCTION; SOLID-STATE CULTIVATION; BETA-MANNANASE; XYLANASE; PURIFICATION; CULTURE;

D O I：

10.5897/AJMR11.1219

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Bacillus sp. HDYM-05 was separated from flax retting liquid and identified as Bacillus cereus which was a new source of microbial mannanase. To optimize the medium components and condition of shake flask experiment for a higher mannanase production, factorial method and response surface methodology were used to obtain the maximal productivity by this species originally. The optimal medium components were: konjaku flour 8.81%, yeast extract 0.5%, NaNO3 0.5%, K2HPO4 0.5%, MgSO4 center dot 7H(2)O 0.02%. The optimal condition of shake flask experiment was: 75 ml medium in 500 ml flask, inoculum volume 1%, initial pH 8.0 and 140 rpm with the cultivation temperature and time at 37 degrees C and 32.05 h, respectively. The maximum enzyme activity of Bacillus sp. HDYM-05 achieved 6777.4 U/ ml, 1.4 folds under the optimal culture conditions as compared to the initial control.

引用

页码：176 / 182

页数：7