Analysis of boron-10 in soft tissue by dynamic secondary ion mass spectrometry

被引:11
作者
Oyedepo, AC
Brooke, SL
Heard, PJ
Day, JCC
Allen, GC
Patel, H
机构
[1] Univ Bristol, Interface Anal Ctr, Bristol BS2 8BS, Avon, England
[2] Queen Elizabeth Hosp, Birmingham B15 2TH, W Midlands, England
[3] Beth Israel Deaconess Med Ctr, Boston, MA 02115 USA
来源
JOURNAL OF MICROSCOPY-OXFORD | 2004年 / 213卷
关键词
boron neutron capture therapy; cultured tumour cells; secondary ion mass spectrometry;
D O I
10.1111/j.1365-2818.2004.01258.x
中图分类号
TH742 [显微镜];
学科分类号
摘要
We report here a preliminary study in which dynamic secondary ion mass spectrometry (SIMS) has provided images of boron-10 (B-10) in biological tissue as used in research into boron neutron capture therapy. Cultured tumour cells incubated in media containing known concentrations of a B-10-containing compound, p-boronophenylalanine (BPA), and intracranial tumour tissue from animals previously injected with BPA were analysed by an in-house constructed SIMS. Investigations were conducted in positive secondary ion detection mode using a 25-keV, 5-nA gallium primary ion source. For calibration purposes, tissue standards were also analysed and their boron-to-carbon signal ratios correlated to bulk boron concentrations measured by inductively coupled plasma atomic emission spectroscopy (ICP-AES). Ion maps of B-10, C-12, Na-23 and K-39 showing gross tissue and cell features were acquired. SIMS and ICP-AES standard measurements were in good agreement. Tissue regions with high or low B-10 concentrations were identified along with B-10 hotspots in normal brain areas. Cultured cells revealed the intracellular localization of B-10. SIMS is capable of producing images showing the distribution of B-10 at p.p.m. levels in cells and in normal and tumour-bearing brain tissue.
引用
收藏
页码:39 / 45
页数:7
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