Alteration of HSF3 and HSP70 mRNA expression in the tissues of two chicken breeds during acute heat stress

被引：17

作者：

Zhang, W. W. ^{[1
,2
,3
]}

Kong, L. N. ^{[1
,2
,3
]}

Zhang, X. Q. ^{[1
,2
,3
]}

Luo, Q. B. ^{[1
,2
,3
]}

机构：

[1] South China Agr Univ, Dept Anim Genet Breeding & Reprod, Coll Anim Sci, Guangzhou, Guangdong, Peoples R China

[2] South China Agr Univ, Key Lab Chicken Genet Breeding & Reprod, Minist Agr, Guangzhou, Guangdong, Peoples R China

[3] Guangdong Prov Key Lab Agroanim Genom & Mol Breed, Guangzhou, Guangdong, Peoples R China

来源：

GENETICS AND MOLECULAR RESEARCH | 2014年 / 13卷 / 04期

基金：

中国国家自然科学基金;

关键词：

Heat shock factor-3; Heat shock protein 70; Chicken; Heat treatment; SHOCK TRANSCRIPTION FACTORS; HEAT-SHOCK-PROTEIN-70; DROSOPHILA; FACTOR-1; PERIODS;

D O I：

10.4238/2014.November.27.6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

This study aimed to estimate changes in HSF3 and HSP70 mRNA expression in stress-sensitive tissues of 2 chicken breeds during acute heat stress. Lingshan chickens (LSC) and White Recessive Rock (WRR) (24 chickens of each breed) were randomly divided into 4 groups (0, 2, 3, and 6 h of heat treatment). With increasing heat treatment time, both HSF3 and HSP70 expression first declined and then showed a significant increase in both breeds. However, HSP70 expression decreased in the heart following 6 h of heat treatment, whereas HSF3 expression continued to increase. After 2 h of heat treatment, HSF3 expression was significantly higher in the brain and leg muscle of LSC compared to WRR (P < 0.05, P < 0.01). In comparison, HSP70 expression was significantly higher in the liver and leg muscle of WRR compared to LSC (P < 0.01, P < 0.05). After 3 h of heat treatment, HSF3 expression was significantly higher in the brain and leg muscle of LSC compared to WRR (P < 0.01). In comparison, HSP70 expression was significantly higher in the liver and heart of LSC compared to WRR (P < 0.01). These results indicate that the expression of HSF3 and HSP70 mRNA in LSC and WRR exhibit species-specific and tissue-specific differences during heat treatment.

引用

页码：9787 / 9794

页数：8

共 19 条

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