CdSe Quantum Dots for Two-Photon Fluorescence Thermal Imaging

被引:282
作者
Martinez Maestro, Laura [1 ]
Martin Rodriguez, Emma [1 ]
Sanz Rodriguez, Francisco [2 ]
Iglesias-de la Cruz, M. C. [2 ]
Juarranz, Angeles [2 ]
Naccache, Rafik [4 ]
Vetrone, Fiorenzo [3 ]
Jaque, Daniel [1 ]
Capobianco, John A. [4 ]
Garcia Sole, Jose [1 ]
机构
[1] Univ Autonoma Madrid, Dept Fis Mat, Fluorescence Imaging Grp, C 4, C Francisco Tomas & Valiente 7, E-28049 Madrid, Spain
[2] Univ Autonoma Madrid, Dept Biol, Madrid 28049, Spain
[3] Univ Quebec, Inst Natl Rech Sci Energie Mat & Telecommun, Varennes, PQ J3X 1S2, Canada
[4] Concordia Univ, Dept Chem & Biochem, Montreal, PQ H4B 1R6, Canada
基金
加拿大自然科学与工程研究理事会;
关键词
Nanothermometry; two-photon microscopy; quantum dots; HeLa cancer cell; thermal sensing; fluorescence imaging; PHOTOLUMINESCENCE; MICROSCOPY; EXCITATION; PROBES; CELLS;
D O I
10.1021/nl1036098
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
The technological development of quantum dots has ushered in a new era in fluorescence bioimaging, which was propelled with the advent of novel multiphoton fluorescence microscopes. Here, the potential use of CdSe quantum dots has been evaluated as fluorescent nanothermometers for two-photon fluorescence microscopy. In addition to the enhancement in spatial resolution inherent to any multiphoton excitation processes, two-photon (near-infrared) excitation leads to a temperature sensitivity of the emission intensity much higher than that achieved under one-photon (visible) excitation. The peak emission wavelength is also temperature sensitive, providing an additional approach for thermal imaging, which is particularly interesting for systems where nanoparticles are not homogeneously dispersed. On the basis of these superior thermal sensitivity properties of the two-photon excited fluorescence, we have demonstrated the ability of CdSe quantum dots to image a temperature gradient artificially created in a biocompatible fluid (phosphate-buffered saline) and also their ability to measure an intracellular temperature increase externally induced in a single living cell.
引用
收藏
页码:5109 / 5115
页数:7
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