Hypoxia-Mediated Down-Regulation of OCTN2 and PPARα Expression in Human Placentas and in BeWo Cells

被引：23
作者：
Chang, Ting-Ting [1 ]
Shyu, Ming-Kwang [2 ]
Huang, Min-Chuan [3 ]
Hsu, Chen-Chi [1 ]
Yeh, Szu-Yu [1 ]
Chen, Mei-Ru [4 ]
Lin, Chun-Jung [1 ]
机构：
[1] Natl Taiwan Univ, Sch Pharm, Coll Med, Taipei 100, Taiwan
[2] Natl Taiwan Univ Hosp, Dept Obstet & Gynecol, Taipei 100, Taiwan
[3] Natl Taiwan Univ, Grad Inst Anat & Cell Biol, Coll Med, Taipei 100, Taiwan
[4] Natl Taiwan Univ, Grad Inst Microbiol, Coll Med, Taipei 100, Taiwan
来源：
MOLECULAR PHARMACEUTICS | 2011年 / 8卷 / 01期
关键词：
OCTN2; PPAR alpha; Preeclampsia; Hypoxia; BeWo cells; ACTIVATED RECEPTOR-ALPHA; INDUCIBLE FACTOR-I; CARNITINE TRANSPORTER; PLASMA CARNITINE; PREECLAMPSIA; DIFFERENTIATION; SLC22A5; INFLAMMATION; METABOLISM; MODULATION;
D O I：
10.1021/mp100137q
中图分类号：
R-3 [医学研究方法]; R3 [基础医学];
学科分类号：
1001 ;
摘要：
The objective of the present study was to investigate the effects of hypoxia on placental expression of OCTN2 and PPAR alpha. OCTN2 and PPAR alpha expression in the human placenta in the presence or absence of preeclampsia was examined by immunohistochemical (IHC) analysis, Western blotting, and quantitative polymerase chain reaction (qPCR). Effects of hypoxia on the expression of OCTN2 and PPAR alpha in human placental explants and human choriocarcinoma BeWo cells were examined by Western blotting and qPCR analyses. IHC, Western blot, and qPCR studies showed that OCTN2 and PPAR alpha protein and mRNA levels were lower in syncytiotrophoblasts from preeclamptic human placentas than in those from normal placentas. Hypoxic treatment caused a decrease in OCTN2 and PPAR alpha expression in human placental explants and in BeWo cells. VVY14643, a PPAR alpha agonist, caused an increase in OCTN2 expression in BeWo cells under hypoxic conditions. In conclusion, under hypoxic conditions, placental OCTN2 is down-regulated through PPAR alpha-mediated pathways.
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收藏
页码：117 / 125
页数：9
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