Enhanced two-electrode photoelectrochemical biosensing platform amplified by bilirubin oxidase labelling

被引：9
作者：
Lu, Yibin [1 ]
Lu, Xueyu [1 ]
Gu, Shiting [1 ]
Shi, Xiao-Mei [2 ]
Fan, Gao-Chao [1 ]
机构：
[1] Qingdao Univ Sci & Technol, Coll Chem & Mol Engn, Shandong Key Lab Biochem Anal, Qingdao 266042, Peoples R China
[2] Nanjing Univ, Sch Chem & Chem Engn, State Key Lab Analyt Chem Life Sci, Nanjing 210093, Peoples R China
来源：
SENSORS AND ACTUATORS B-CHEMICAL | 2021年 / 343卷
基金：
中国国家自然科学基金;
关键词：
Photoelectrochemistry; Two-electrode; Biosensing; Bilirubin oxidase; CEA; CARCINOEMBRYONIC ANTIGEN; AMPLIFICATION STRATEGY; SIGNAL AMPLIFICATION; ENERGY-TRANSFER; IMMUNOASSAY; IMMUNOSENSOR; NANOPARTICLES; APTASENSOR; CYTOSENSOR; DNAZYME;
D O I：
10.1016/j.snb.2021.130060
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Herein, a novel and robust two-electrode photoelectrochemical (PEC) biosensing platform amplified by bilirubin oxidase (BOD) labelling was reported. This two-electrode operating system was composed of photoanode and sensing biocathode. Carcinoembryonic antigen (CEA) was utilized as a model for probing. Specifically, Fe3+doped TiO2 (Fe:TiO2) nanotubes were first prepared and then deposited with ZnIn2S4 (ZIS) nanocrystals to develop the ZIS/Fe:TiO2 photoanode. Toward sensing biocathode, after carbon nanotubes (CNTs) and Au nanoparticles (NPs) were modified on conductive glass, an Au/CNT cathodic substrate was fabricated to anchor the CEA aptamer. Complementary DNA (cDNA) of the CEA aptamer was labelled with BOD to form the cDNABOD conjugate that amplified the signal through DNA hybridization. Target detection hinged on an evident decline in the current signal generated by terminated amplification of the BOD labels and evident steric hindrance of the captured CEA molecules. Profiting from excellent PEC property of the ZIS/Fe:TiO2 photoanode and good oxygen-reduction capability of the BOD labels, the two-electrode PEC biosensing platform exhibited high sensitivity. Because of the division of biorecognition with photoanode, the platform also showed satisfactory selectivity in biological matrix. This elegant two-electrode strategy offers a promising path for seeking other robust PEC biosensors applying in complex biological samples.
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页数：9
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共 51 条
[1]   Hybrid PbS Quantum Dot/Nanoporous NiO Film Nanostructure: Preparation, Characterization, and Application for a Self-Powered Cathodic Photoelectrochemical Biosensor [J].
Dai, Wen-Xia ;
Zhang, Ling ;
Zhao, Wei-Wei ;
Yu, Xiao-Dong ;
Xu, Jing-Juan ;
Chen, Hong-Yuan .
ANALYTICAL CHEMISTRY, 2017, 89 (15) :8070-8078
[2]   Surface-enhanced electrochemiluminescence combined with resonance energy transfer for sensitive carcinoembryonic antigen detection in exhaled breath condensates [J].
Ding, Li ;
Xu, Shaohua ;
Huang, Dandan ;
Chen, Lifen ;
Kannan, Palanisamy ;
Guo, Longhua ;
Lin, Zhenyu .
ANALYST, 2020, 145 (20) :6524-6531
[3]   Mechanistic studies of the 'blue' Cu enzyme, bilirubin oxidase, as a highly efficient electrocatalyst for the oxygen reduction reaction [J].
dos Santos, Luciano ;
Climent, Victor ;
Blanford, Christopher F. ;
Armstrong, Fraser A. .
PHYSICAL CHEMISTRY CHEMICAL PHYSICS, 2010, 12 (42) :13962-13974
[4]   Bilirubin oxidase from Bacillus pumilus: A promising enzyme for the elaboration of efficient cathodes in biofuel cells [J].
Durand, Fabien ;
Kjaergaard, Christian Hauge ;
Suraniti, Emmanuel ;
Gounel, Sebastien ;
Hadt, Ryan G. ;
Solomon, Edward I. ;
Mano, Nicolas .
BIOSENSORS & BIOELECTRONICS, 2012, 35 (01) :140-146
[5]   Polydopamine-PEG-Folic Acid Conjugate Film Engineered TiO2 Nanotube Arrays for Photoelectrochemical Sensing of Folate Binding Protein [J].
Fan, Bobo ;
Fan, Qi ;
Hu, Lulin ;
Cui, Min ;
Wang, Xueying ;
Ma, Hongmin ;
Wei, Qin .
ACS APPLIED MATERIALS & INTERFACES, 2020, 12 (01) :1877-1884
[6]   Facile fabrication of an aptasensor for thrombin based on graphitic carbon nitride/TiO2 with high visible-light photoelectrochemical activity [J].
Fan, Dawei ;
Guo, Cuijuan ;
Ma, Hongmin ;
Zhao, Di ;
Li, Yina ;
Wu, Dan ;
Wei, Qin .
BIOSENSORS & BIOELECTRONICS, 2016, 75 :116-122
[7]   An ultrasensitive label-free immunosensor based on CdS sensitized Fe-TiO2 with high visible-light photoelectrochemical activity [J].
Fan, Dawei ;
Wu, Dan ;
Cui, Jiali ;
Chen, Yucheng ;
Ma, Hongmin ;
Liu, Yixin ;
Wei, Qin ;
Du, Bin .
BIOSENSORS & BIOELECTRONICS, 2015, 74 :843-848
[8]   Robust photoelectrochemical cytosensor in biological media using antifouling property of zwitterionic peptide [J].
Fan, Gao-Chao ;
Li, Zimeng ;
Lu, Yanwei ;
Ma, Linzheng ;
Zhao, Huan ;
Luo, Xiliang .
SENSORS AND ACTUATORS B-CHEMICAL, 2019, 299
[9]   Separating photoanode from recognition events: toward a general strategy for a self-powered photoelectrochemical immunoassay with both high sensitivity and anti-interference capabilities [J].
Fan, Gao-Chao ;
Ma, Linzheng ;
Jayachandran, Silambarasan ;
Li, Zimeng ;
Luo, Xiliang .
CHEMICAL COMMUNICATIONS, 2018, 54 (51) :7062-7065
[10]   Cathode Photoelectrochemical Immunosensing Platform Integrating Photocathode with Photoanode [J].
Fan, Gao-Chao ;
Shi, Xiao-Mei ;
Zhang, Jian-Rong ;
Zhu, Jun-Jie .
ANALYTICAL CHEMISTRY, 2016, 88 (21) :10352-10356
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