High-yield production of biologically active recombinant protein in shake flask culture by combination of enzyme-based glucose delivery and increased oxygen transfer

被引：42

作者：

Ukkonen, Kaisa ^{[1
,2
]}

Vasala, Antti ^{[1
]}

Ojamo, Heikki ^{[2
]}

Neubauer, Peter ^{[3
]}

机构：

[1] BioSilta Cy, FI-90014 Oulu, Finland

[2] Univ Oulu, Dept Proc & Environm Engn, Bioproc Engn Lab, FI-90014 Oulu, Finland

[3] Tech Univ Berlin, Chair Bioproc Engn, Dept Biotechnol, D-13355 Berlin, Germany

来源：

MICROBIAL CELL FACTORIES | 2011年 / 10卷

关键词：

Shake flask; Oxygen transfer; Recombinant protein; Fed-batch; HIGH CELL-DENSITY; ESCHERICHIA-COLI; RIBONUCLEASE INHIBITOR; SCALE-UP; CULTIVATION; EXPRESSION;

D O I：

10.1186/1475-2859-10-107

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

This report describes the combined use of an enzyme-based glucose release system (EnBase (R)) and high-aeration shake flask ( Ultra Yield Flask T). The benefit of this combination is demonstrated by over 100-fold improvement in the active yield of recombinant alcohol dehydrogenase expressed in E. coli. Compared to Terrific Broth and ZYM-5052 autoinduction medium, the EnBase system improved yield mainly through increased productivity per cell. Four-fold increase in oxygen transfer by the Ultra Yield Flask contributed to higher cell density with EnBase but not with the other tested media, and consequently the product yield per ml of EnBase culture was further improved.

引用

页数：9

共 17 条

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