Small extracellular vesicles from iPSC-derived mesenchymal stem cells ameliorate tendinopathy pain by inhibiting mast cell activation

被引：25

作者：

Gao, Renzhi ^{[1
,2
]}

Ye, Teng ^{[1
,2
]}

Zhu, Zhaochen ^{[1
,2
]}

Li, Qing ^{[1
]}

Zhang, Juntao ^{[1
]}

Yuan, Ji ^{[1
]}

Zhao, Bizeng ^{[2
]}

Xie, Zongping ^{[2
]}

Wang, Yang ^{[1
]}

机构：

[1] Shanghai Jiao Tong Univ, Inst Microsurg Extrem, Affiliated Peoples Hosp 6, 600 Yishan Rd, Shanghai 200233, Peoples R China

[2] Shanghai Jiao Tong Univ, Dept Orthopaed Surg, Affiliated Peoples Hosp 6, 600 Yishan Rd, Shanghai 200233, Peoples R China

来源：

NANOMEDICINE | 2022年 / 17卷 / 08期

基金：

中国国家自然科学基金;

关键词：

iMSC-sEV; mast cell; pain; tendinopathy; HYPERSENSITIVITY; DEGRANULATION; SENSITIZATION; MSC;

D O I：

10.2217/nnm-2022-0036

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Aim: This study aimed to explore the effect of small extracellular vesicles from induced pluripotent stem cell-derived mesenchymal stem cells (iMSC-sEVs) on acute pain and investigate the underlying mechanisms. Materials & methods: The pathology of tendons was accessed by hematoxylin and eosin staining, immunohistochemical and immunofluorescent staining. The pain degree was measured by pain-related behaviors. In vitro, we performed beta-hexosaminidase release assay, RT-qPCR, toluidine blue staining, ELISA and RNA sequencing. Results: iMSC-sEVs effectively alleviated acute pain in tendinopathy as well as inhibiting activated mast cell infiltration and interactions with nerve fibers in vivo. In vitro, iMSC-sEVs reduced the degranulation of mast cells and the expression of proinflammatory cytokines and genes involved in the HIF-1 signaling pathway. Conclusion: This study demonstrated that iMSC-sEVs relieved tendinopathy-related pain through inhibiting mast cell activation via the HIF-1 signaling pathway.

引用

页码：513 / 529

页数：17

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