Dose-dependent micronuclei formation in normal human fibroblasts exposed to proton radiation

被引：11

作者：

Litvinchuk, Alexandra V. ^{[1
]}

Vachelova, J. ^{[1
]}

Michaelidesova, A. ^{[1
,2
]}

Wagner, R. ^{[1
]}

Davidkova, M. ^{[1
]}

机构：

[1] Nucl Phys Inst CAS, Dept Radiat Dosimetry, Prague 18000, Czech Republic

[2] Proton Therapy Ctr, Prague 18000, Czech Republic

来源：

RADIATION AND ENVIRONMENTAL BIOPHYSICS | 2015年 / 54卷 / 03期

关键词：

Human fibroblasts; Proton radiation; Micronuclei assay; Biodosimetry; PERIPHERAL-BLOOD LYMPHOCYTES; CULTURED HUMAN-CELLS; IONIZING-RADIATION; DNA-DAMAGE; RETROSPECTIVE DOSIMETRY; CHROMOSOME-ABERRATIONS; BIOLOGICAL DOSIMETER; PROJECT PERSPECTIVE; EPITHELIAL-CELLS; BUCCAL CELLS;

D O I：

10.1007/s00411-015-0598-1

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Micronuclei are small extranuclear bodies resulting from chromosome fragments or the whole chromosomes secluded from daughter nuclei during mitosis. The number of radiation-induced micronuclei reflects the level of chromosomal damage and relates to an absorbed dose and quality of incident ionizing radiation. The aim of the present study was to determine the micronucleus formation as a specific biological marker for acute radiation-induced DNA damage in normal human fibroblasts exposed to 30-MeV protons and Co-60 gamma radiation. We found a linear increase in binuclear cells containing micronuclei for absorbed doses from 1 to 5 Gy for both radiation modalities. However, the total number of micronuclei in binuclear cells follows a linear-quadratic dose dependence. In case of human exposure to mixed radiation fields or high LET radiation, the proportion of binuclear cells containing micronuclei from all binuclear cells can thus serve as a good biomarker of radiation-induced DNA damage.

引用

页码：327 / 334

页数：8

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