Dual-Label STED Nanoscopy of Living Cells Using Photochromism

被引：47

作者：

Willig, Katrin I. ^{[1
]}

Stiel, Andre C. ^{[1
]}

Brakemann, Tanja ^{[1
]}

Jakobs, Stefan ^{[1
]}

Hell, Stefan W. ^{[1
]}

机构：

[1] Max Planck Inst Biophys Chem, D-37077 Gottingen, Germany

来源：

NANO LETTERS | 2011年 / 11卷 / 09期

关键词：

Microscopy; two-color; superresolution; photoswitching; green fluorescent protein; RSFP; STIMULATED-EMISSION; FLUORESCENCE MICROSCOPY; PROTEINS;

D O I：

10.1021/nl202290w

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We demonstrate live-cell STED microscopy of two protein species using photochromic green fluorescent proteins as markers. The reversible photoswitching of two markers is implemented so that they can be discerned with a single excitation and STED wavelength and a single detection channel. Dual-label STED microscopy is shown in living mammalian cells.

引用

页码：3970 / 3973

页数：4

共 17 条

[1] Regulated fast nucleocytoplasmic shuttling observed by reversible protein highlighting [J].

Ando, R ;

Mizuno, H ;

Miyawaki, A .

SCIENCE, 2004, 306 (5700) :1370-1373

[2] Photoswitchable fluorescent proteins enable monochromatic multilabel imaging and dual color fluorescence nanoscopy [J].

Andresen, Martin ;

Stiel, Andre C. ;

Foelling, Jonas ;

Wenzel, Dirk ;

Schoenle, Andreas ;

Egner, Alexander ;

Eggeling, Christian ;

Hell, Stefan W. ;

Jakobs, Stefan .

NATURE BIOTECHNOLOGY, 2008, 26 (09) :1035-1040

[3] Simultaneous multi-lifetime multi-color STED imaging for colocalization analyses [J].

Bueckers, Johanna ;

Wildanger, Dominik ;

Vicidomini, Giuseppe ;

Kastrup, Lars ;

Hell, Stefan W. .

OPTICS EXPRESS, 2011, 19 (04) :3130-3143

[4] Two-color far-field fluorescence nanoscopy [J].

Donnert, Gerald ;

Keller, Jan ;

Wurm, Christian A. ;

Rizzoli, Silvio O. ;

Westphal, Volker ;

Schoenle, Andreas ;

Jahn, Reinhard ;

Jakobs, Stefan ;

Eggeling, Christian ;

Hell, Stefan W. .

BIOPHYSICAL JOURNAL, 2007, 92 (08) :L67-L69

[5] STED Nanoscopy in Living Cells Using Fluorogen Activating Proteins [J].

Fitzpatrick, James A. J. ;

Yan, Qi ;

Sieber, Jochen J. ;

Dyba, Marcus ;

Schwarz, Ulf ;

Szent-Gyorgyi, Chris ;

Woolford, Carol A. ;

Berget, Peter B. ;

Waggoner, Alan S. ;

Bruchez, Marcel P. .

BIOCONJUGATE CHEMISTRY, 2009, 20 (10) :1843-1847

[6] Characterization via Two-Color STED Microscopy of Nanostructured Materials Synthesized by Colloid Electrospinning [J].

Friedemann, Kathrin ;

Turshatov, Andrey ;

Landfester, Katharina ;

Crespy, Daniel .

LANGMUIR, 2011, 27 (11) :7132-7139

[7]

Gugel H., 2011, P SPIE, P7905

[8] Stimulated Emission Depletion Nanoscopy of Living Cells Using SNAP-Tag Fusion Proteins [J].

Hein, Birka ;

Willig, Katrin I. ;

Wurm, Christian A. ;

Westphal, Volker ;

Jakobs, Stefan ;

Hell, Stefan W. .

BIOPHYSICAL JOURNAL, 2010, 98 (01) :158-163

[9]

Hell SW, 2009, NAT METHODS, V6, P24, DOI [10.1038/NMETH.1291, 10.1038/nmeth.1291]

[10] BREAKING THE DIFFRACTION RESOLUTION LIMIT BY STIMULATED-EMISSION - STIMULATED-EMISSION-DEPLETION FLUORESCENCE MICROSCOPY [J].

HELL, SW ;

WICHMANN, J .

OPTICS LETTERS, 1994, 19 (11) :780-782

← 1 2 →