Structural Characterization and Immunostimulatory Activity of Polysaccharides from Brassica rapa L.

被引：78

作者：

Chen, Zhuo-Er ^{[1
]}

Wufuer, Reziyamu ^{[1
]}

Ji, Jin-Hu ^{[2
]}

Li, Jin-Fang ^{[3
]}

Cheng, Yu-Feng ^{[1
]}

Dong, Cai-Xia ^{[4
]}

Taoerdahong, Hailiqian ^{[1
]}

机构：

[1] Xinjiang Med Univ, Coll Pharm, Urumqi, Xinjiang Uygur, Peoples R China

[2] Xinjiang Med Univ, Med Engn Technol Inst, Urumqi, Xinjiang Uygur, Peoples R China

[3] Xinjiang Med Univ, Coll HouBo, Expt Teach Ctr, Karamay, Xinjiang Uygur, Peoples R China

[4] Tianjin Med Univ, Sch Pharm, Tianjin Key Lab Technol Enabling Dev Clin Therape, Tianjin 300070, Peoples R China

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2017年 / 65卷 / 44期

基金：

中国国家自然科学基金;

关键词：

Brassica rapa L; polysaccharides; structural characterization; immunostimulatory activity; ANTIOXIDANT ACTIVITY; ANTITUMOR-ACTIVITY; IN-VITRO; PURIFICATION; MACROPHAGE; OPTIMIZATION; INFLAMMATION; DERIVATIVES; EXTRACTION; CANCER;

D O I：

10.1021/acs.jafc.7b03902

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

Two neutral polysaccharides (BRNP-1, 6.9 kDa; BRNP-2, 4.8 kDa) were purified from the common edible plant Brassica rapa L. via the combined techniques of ion-exchange chromatography and high-performance gel permeation chromatography. Monosaccharide composition analysis showed that BRNP-1 and BRNP-2 were composed of glucosyl residues. Methylation and 1D- and 2D-NMR analyses revealed that both BRNP-1 and BRNP-2 contained a backbone chain that was composed of alpha-D-(1 -> 4)-linked Glcp residues and side chains that were composed of terminally linked Glcp residues attached at the O-6 position of backbone-glycosyl residues. BRNP-1 and BRNP-2, however, differed in branch degree and molecular weight. Bioassay results showed that treatment with the higher dosage (400,mu g/mL) of BRNP-1 and BRNP-2 stimulated the proliferation, NO release, and cytokine secretion (IL-6 and TNF-alpha) of RAW264.7 macrophages. These results suggested that BRNP-1 and BRNP-2 may enhance macrophage-mediated immune responses.

引用

页码：9685 / 9692

页数：8

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