cDNA-AFLP analysis of differential gene expression related to cell chemotactic and encystment of Azospirillum brasilense

被引：4
|
作者：
Li, Huamin [1 ,2 ]
Cui, Yanhua [1 ,2 ,3 ]
Wu, Lixian [1 ,2 ]
Tu, Ran [1 ,2 ]
Chen, Sanfeng [1 ,2 ]
机构：
[1] China Agr Univ, State Key Lab Agrobiotechnol, Beijing 100193, Peoples R China
[2] China Agr Univ, Coll Biol Sci, Beijing 100193, Peoples R China
[3] Harbin Inst Technol, Sch Food Sci & Engn, Harbin 150090, Peoples R China
来源：
MICROBIOLOGICAL RESEARCH | 2011年 / 166卷 / 08期
关键词：
cDNA-AFLP; Chemotaxis; Encystment; Nitrogen fixation; Azospirillum brosilense; RHODOBACTER-CAPSULATUS; NITROGENASE ACTIVITY; FUNCTIONAL-ANALYSIS; REGULATORY SYSTEM; NIFA; OXIDASE; PROTEINS; COMPLEX; PLANT; POLYHYDROXYALKANOATES;
D O I：
10.1016/j.micres.2010.11.007
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Our previous study indicated org35 was involved in chemotaxis and interacted with nitrogen fixation transcriptional activator NifA via PAS domain. In order to reveal the role of org35 in nitrogen regulation, the downstream target genes of org35 were identified. We here report differentially expressed genes in org35 mutants comparing with wild type Sp7 by means of cDNA-AFLP. Four up-regulated transcript-derived fragments (TDFs) homologues of chemotaxis transduction proteins were found, including CheW, methyl-accepting chemotaxis protein and response regulator CheY-like receiver. Three distinct TDFs (AB46, AB58 and AB63) were similar to PHB de-polymerase C-terminus, cell shape-determining protein and flagellin domain protein. And 11 TDFs showed similarities with signal transduction proteins, including homologous protein of the nitrogen regulation protein NtrY and nitrate/nitrite response regulator protein NarL. These data suggested that the Azospirillum brasilense org35 was a multi-effecter and involved in chemotaxis, cyst development and regulation of nitrogen fixation. (C) 2010 Elsevier GmbH. All rights reserved.
引用
收藏
页码：595 / 605
页数：11
相关论文
共 50 条
  • [21] cDNA-AFLP Analysis Reveals Differential Gene Expression in Response to Salt Stress in Foxtail Millet (Setaria italica L.)
    Jayaraman, Ananthi
    Puranik, Swati
    Rai, Neeraj Kumar
    Vidapu, Sudhakar
    Sahu, Pranav Pankaj
    Lata, Charu
    Prasad, Manoj
    MOLECULAR BIOTECHNOLOGY, 2008, 40 (03) : 241 - 251
  • [22] Quantitative cDNA-AFLP analysis for genome-wide expression studies
    Breyne, P
    Dreesen, R
    Cannoot, B
    Rombaut, D
    Vandepoele, K
    Rombauts, S
    Vanderhaeghen, R
    Inzé, D
    Zabeau, M
    MOLECULAR GENETICS AND GENOMICS, 2003, 269 (02) : 173 - 179
  • [23] Differential gene expression in Azospirillum brasilense Cd under saline stress
    Jofré, E
    Rivarola, V
    Balegno, H
    Mori, G
    CANADIAN JOURNAL OF MICROBIOLOGY, 1998, 44 (10) : 929 - 936
  • [24] Quantitative cDNA-AFLP analysis for genome-wide expression studies
    P. Breyne
    R. Dreesen
    B. Cannoot
    D. Rombaut
    K. Vandepoele
    S. Rombauts
    R. Vanderhaeghen
    D. Inzé
    M. Zabeau
    Molecular Genetics and Genomics, 2003, 269 : 173 - 179
  • [25] Differential gene expression in incompatible interaction between wheat and stripe rust fungus revealed by the cDNA-AFLP technique
    Wang, X.
    Ma, J.
    Li, Y.
    Chen, X.
    Huang, X.
    Han, Q.
    Wei, G.
    Huang, L.
    Kang, Z.
    PHYTOPATHOLOGY, 2009, 99 (06) : S138 - S138
  • [26] Expression analysis of stem prickle formation in blackberry using cDNA-AFLP
    Zhang, C. H.
    Wu, W. L.
    Hu, S. Y.
    Lv, L. F.
    Li, W. L.
    XXIX INTERNATIONAL HORTICULTURAL CONGRESS ON HORTICULTURE: SUSTAINING LIVES, LIVELIHOODS AND LANDSCAPES (IHC2014): INTERNATIONAL SYMPOSIUM ON MOLECULAR BIOLOGY IN HORTICULTURE, 2016, 1110 : 171 - 177
  • [27] cDNA-AFLP technique discloses differential gene expression in response to salinity in wheat (Triticum aestivum L.)
    Kamyab, Shabnam
    Alami-Saeid, Khalil
    Eslahi, Mohammadreza
    Moradi, Mohammad
    GENETIC RESOURCES AND CROP EVOLUTION, 2021, 68 (06) : 2299 - 2311
  • [28] cDNA-AFLP technique discloses differential gene expression in response to salinity in wheat (Triticum aestivum L.)
    Shabnam Kamyab
    Khalil Alami-Saeid
    Mohammadreza Eslahi
    Mohammad Moradi
    Genetic Resources and Crop Evolution, 2021, 68 : 2299 - 2311
  • [29] AFLP-based transcript profiling (cDNA-AFLP) for genome-wide expression analysis
    Vuylsteke, Marnik
    Peleman, Johan D.
    van Eijk, Michiel J. T.
    NATURE PROTOCOLS, 2007, 2 (06) : 1399 - 1413
  • [30] AFLP-based transcript profiling (cDNA-AFLP) for genome-wide expression analysis
    Marnik Vuylsteke
    Johan D Peleman
    Michiel JT van Eijk
    Nature Protocols, 2007, 2 : 1399 - 1413
12345