Chronic stress inhibits testosterone synthesis in Leydig cells through mitochondrial damage via Atp5a1

被引：15

作者：

Xiong, Xiaofan ^{[1
,2
]}

Wu, Qiuhua ^{[3
,4
]}

Zhang, Lingyu ^{[2
]}

Gao, Shanfeng ^{[2
]}

Li, Rufeng ^{[2
]}

Han, Lin ^{[3
]}

Fan, Meiyang ^{[2
]}

Wang, Miaomiao ^{[2
]}

Liu, Liying ^{[3
]}

Wang, Xiaofei ^{[3
]}

Zhang, Chunli ^{[1
]}

Xin, Yanlong ^{[1
]}

Li, Zongfang ^{[1
]}

Huang, Chen ^{[2
,3
]}

Yang, Juan ^{[2
,3
]}

机构：

[1] Xi An Jiao Tong Univ, Western China Sci & Technol Innovat Port Precis M, Affiliated Hosp 2, Xian, Peoples R China

[2] Xi An Jiao Tong Univ, Hlth Sci Ctr, Sch Basic Med Sci, Dept Cell Biol & Genet, Xian 710061, Peoples R China

[3] Xi An Jiao Tong Univ, Minist Educ China, Key Lab Environm & Genes Related Dis, Xian, Peoples R China

[4] Northwest Womens & Childrens Hosp, Ctr Med Genet, Xian, Peoples R China

来源：

JOURNAL OF CELLULAR AND MOLECULAR MEDICINE | 2022年 / 26卷 / 02期

基金：

美国国家科学基金会;

关键词：

Atp5a1; Leydig cells; mitochondrial dysfunction; testosterone synthesis; M2; PYRUVATE-KINASE; PSYCHOLOGICAL STRESS; OXIDATIVE STRESS; BINDING PARTNERS; GENE-EXPRESSION; PROTEINS; SPERMATOGENESIS; IDENTIFICATION; INFLAMMATION; PATIENT;

D O I：

10.1111/jcmm.17085

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Stress is one of the leading causes of male infertility, but its exact function in testosterone synthesis has scarcely been reported. We found that adult male rats show a decrease in bodyweight, genital index and serum testosterone level after continual chronic stress for 21 days. Two-dimensional gel electrophoresis (2-DE) and MALDI-TOF-MS analysis identified 10 differentially expressed proteins in stressed rats compared with controls. A strong protein interaction network was found to be centred on Atp5a1 among these proteins. Atp5a1 expression significantly decreased in Leydig cells after chronic stress. Transfection of Atp5a1 siRNAs decreased StAR, CYP11A1, and 17 beta-HSD expression by damaging the structure of mitochondria in TM3 cells. This study confirmed that chronic stress plays an important role in testosterone synthesis by regulating Atp5a1 expression in Leydig cells.

引用

页码：354 / 363

页数：10

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