Performance of cytochrome c oxidase subunit I (COI), ribosomal DNA Large Subunit (LSU) and Internal Transcribed Spacer 2 (ITS2) in DNA barcoding of Collembola

被引：32

作者：

Anslan, Sten ^{[1
]}

Tedersoo, Leho ^{[2
]}

机构：

[1] Univ Tartu, Inst Ecol & Earth Sci, EE-50411 Tartu, Estonia

[2] Univ Tartu, Nat Hist Museum, EE-50411 Tartu, Estonia

来源：

EUROPEAN JOURNAL OF SOIL BIOLOGY | 2015年 / 69卷

关键词：

Springtails; Molecular identification; Internal transcribed spacer (ITS); Cytochrome c oxidase subunit I (COI; CO1; cox1; coxI); Ribosomal large subunit (LSU; 28S); DNA barcoding; BIODIVERSITY ASSESSMENT; SEQUENCE ALIGNMENT; CRYPTIC DIVERSITY; SOIL; IDENTIFICATION; HEXAPODA; SCALE;

D O I：

10.1016/j.ejsobi.2015.04.001

中图分类号：

Q14 [生态学（生物生态学）];

学科分类号：

071012 ; 0713 ;

摘要：

Studies in soil meiofauna with traditional methods are highly laborious and identification exclusively by means of morphological traits usually underestimates species richness. Although the mitochondrial DNA Cytochrome c Oxidase subunit I gene (COI, CO1, coxl, coxI) has been proposed as a standard DNA barcode for animals, nuclear ribosomal DNA markers such as Large Subunit (LSU, 28S) and Internal Transcribed Spacer (ITS) have proven efficient to distinguish among species of animals, eukaryotic microbes and plants. The COI and LSU markers discriminate among closely related species of Collembola, but the efficiency of ITS2 has never been tested. We evaluated the relative performance of these three markers for DNA barcoding of Collembola. All three markers proved highly efficient in discriminating species, but LSU exhibited very low differences among certain closely related species. We conclude that ITS2 may serve as an alternative barcode to identify Collembola and has a potential to be used for metabarcoding analyses. (C) 2015 Elsevier Masson SAS. All rights reserved.

引用

页码：1 / 7

页数：7

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