Immobilization of barley oxalate oxidase onto gold-nanoparticle-porous CaCO3 microsphere hybrid for amperometric determination of oxalate in biological materials

被引：19

作者：

Chauhan, Nidhi ^{[1
]}

Narang, Jagriti ^{[1
]}

Shweta ^{[1
]}

Pundir, C. S. ^{[1
]}

机构：

[1] MD Univ, Dept Biochem, Rohtak 124001, Haryana, India

来源：

CLINICAL BIOCHEMISTRY | 2012年 / 45卷 / 03期

关键词：

Oxalate; Oxalate oxidase; Urine; Plasma; Enzyme electrode; URINARY OXALATE; DIRECT ELECTROCHEMISTRY; PLASMA OXALATE; BIOSENSOR;

D O I：

10.1016/j.clinbiochem.2011.12.004

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

Objectives: The use of Au nanoparticles (AuNPs)-CaCO3 porous microsphere hybrid as a matrix for enzyme immobilization to increase the performance of an amperometric biosensor. Design and methods: A method is described for construction of an amperometric oxalate biosensor by immobilizing a barley root oxalate oxidase (OxOx) onto AuNPs-CaCO3 porous microsphere hybrid encapsulated in silica sol and deposited on Au electrode. Results: The biosensor showed optimum response within 4 s at pH 5.0 and 30 degrees C, when polarized at +0.4 V vs. Ag/agCl. The biosensor possesses high sensitivity and measures oxalate concentrations as low as 1.0 mu mol/L. The working linear range was from 1.0 to 1000 mu mol/L of oxalate. The biosensor measured urinary and plasma oxalate of normal persons and stone formers. Conclusion: The use of AuNPs-CaCO3 porous microsphere hybrid as a support for immobilization of OxOx has resulted into an improved amperometric oxalate biosensor. (C) 2011 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.

引用

页码：253 / 258

页数：6

共 27 条

[1]

Amini AS, 1994, ANAL CHIM ACTA, V299, P75

[2] Studies of the binding and signaling of surface-immobilized periplasmic glucose receptors on gold nanoparticles: A glucose biosensor application [J].

Andreescu, Silvana ;

Luck, Linda A. .

ANALYTICAL BIOCHEMISTRY, 2008, 375 (02) :282-290

[3]

ARCHER HE, 1957, CLIN SCI, V16, P405

[4]

Balion Cynthia M., 1994, Clinical Chemistry, V40, P1096

[5]

BIAS R, 1980, ANAL CHEM, V52, P508

[6] Porous gold-nanoparticle-CaCO3 hybrid material:: Preparation, characterization, and application for horseradish peroxidase assembly and direct electrochemistry [J].

Cai, WY ;

Xu, Q ;

Zhao, XN ;

Zhu, JJ ;

Chen, HY .

CHEMISTRY OF MATERIALS, 2006, 18 (02) :279-284

[7]

Chaudhary R., 2010, SENS T, V113, P127

[8]

Chiraboga J., 1965, ARCH BIOCHEM BIOPHYS, V116, P516

[9] SIMPLIFIED METHOD FOR ENZYMATIC URINE OXALATE ASSAY [J].

CRIDER, QE ;

CURRAN, DF .

CLINICAL BIOCHEMISTRY, 1984, 17 (06) :351-355

[10]

EARNEST DL, 1974, GASTROENTEROLOGY, V66, P1114

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