Identification and genomic characterization of major effect bacterial blight resistance locus (BB-13) in Upland cotton (Gossypium hirsutum L.)

被引:8
|
作者
Gowda, S. Anjan [1 ]
Shrestha, Navin [1 ]
Harris, Taylor M. [2 ,4 ]
Phillips, Anne Z. [2 ]
Fang, Hui [1 ]
Sood, Shilpa [1 ]
Zhang, Kuang [1 ]
Bourland, Fred [3 ]
Bart, Rebecca [2 ]
Kuraparthy, Vasu [1 ]
机构
[1] North Carolina State Univ, Crop & Soil Sci Dept, Raleigh, NC 27695 USA
[2] Donald Danforth Plant Sci Ctr, 975 N Warson Rd, St Louis, MO 63132 USA
[3] Univ Arkansas, Crop Soil & Environm Sci, NE Res & Extens Ctr, Keiser, AR 72351 USA
[4] Washington Univ, Div Biol & Biomed Sci, St Louis, MO 63110 USA
基金
美国国家科学基金会;
关键词
DISEASE RESISTANCE; BLACKARM RESISTANCE; POPULATION-STRUCTURE; GENE; SOFTWARE; MAP; REGISTRATION; INHERITANCE; SEQUENCE; QUALITY;
D O I
10.1007/s00122-022-04229-2
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
Key message Identification and genomic characterization of major resistance locus against cotton bacterial blight (CBB) using GWAS and linkage mapping to enable genomics-based development of durable CBB resistance and gene discovery in cotton. Cotton bacterial leaf blight (CBB), caused by Xanthomonas citri subsp. malvacearum (Xcm), has periodically been a damaging disease in the USA. Identification and deployment of genetic resistance in cotton cultivars is the most economical and efficient means of reducing crop losses due to CBB. In the current study, genome-wide association study (GWAS) of CBB resistance using an elite diversity panel of 380 accessions, genotyped with the cotton single nucleotide polymorphism (SNP) 63 K array, and phenotyped with race-18 of CBB, localized the CBB resistance to a 2.01-Mb region in the long arm of chromosome D02. Molecular genetic mapping using an F-6 recombinant inbred line (RIL) population showed the CBB resistance in cultivar Arkot 8102 was controlled by a single locus (BB-13). The BB-13 locus was mapped within the 0.95-cM interval near the telomeric region in the long arm of chromosome D02. Flanking SNP markers, i04890Gh and i04907Gh of the BB-13 locus, identified from the combined linkage analysis and GWAS, targeted it to a 371-Kb genomic region. Candidate gene analysis identified thirty putative gene sequences in the targeted genomic region. Nine of these putative genes and two NBS-LRR genes adjacent to the targeted region were putatively involved in plant disease resistance and are possible candidate genes for BB-13 locus. Genetic mapping and genomic targeting of the BB13 locus in the current study will help in cloning the CBB-resistant gene and establishing the molecular genetic architecture of the BB-13 locus towards developing durable resistance to CBB in cotton.
引用
收藏
页码:4421 / 4436
页数:16
相关论文
共 50 条
  • [41] Identification and Functional Characterization of a Microtubule-Associated Protein, GhCLASP2, From Upland Cotton (Gossypium hirsutum L.)
    Zhu, Shou-Hong
    Xue, Fei
    Li, Yan-Jun
    Liu, Feng
    Zhang, Xin-Yu
    Zhao, Lan-Jie
    Sun, Yu-Qiang
    Zhu, Qian-Hao
    Sun, Jie
    FRONTIERS IN PLANT SCIENCE, 2018, 9
  • [42] Development and Characterization of a Cotton (Gossypium hirsutum L.) Event with Enhanced Reproductive Resistance to Glyphosate
    Cerny, R. Eric
    Bookout, Jeffrey T.
    CaJacob, Claire A.
    Groat, Jeanna R.
    Hart, Jesse L.
    Heck, Greg R.
    Huber, Scott A.
    Listello, Jennifer
    Martens, Amy B.
    Oppenhuizen, Mark E.
    Sammons, Bernard
    Scanlon, Niki K.
    Shappley, Zachary W.
    Yang, Julie X.
    Xiao, Jinhua
    CROP SCIENCE, 2010, 50 (04) : 1375 - 1384
  • [43] The effect of the non ionizing radiation on exposed, laboratory cultivated upland cotton (Gossypium hirsutum L.) plants
    Stefi, Aikaterina L.
    Margaritis, Lukas H.
    Christodoulakis, Nikolaos S.
    FLORA, 2017, 226 : 55 - 64
  • [44] EFFECT OF SUBTENDING LEAF REMOVAL ON THE YIELD AND FIBER QUALITY TRAITS OF UPLAND COTTON (GOSSYPIUM HIRSUTUM L.)
    Mangi, N.
    Ma, Q.
    Jatoi, G. H.
    Sarfraz, Z.
    Soomro, N.
    Iqbal, M. S.
    Wang, X.
    Jarwar, A. H.
    Shuli, F.
    APPLIED ECOLOGY AND ENVIRONMENTAL RESEARCH, 2021, 19 (04): : 3203 - 3225
  • [45] Effect of Implantation Machine Parameters on N~+ ion Implantation for Upland Cotton(Gossypium hirsutum L.)Pollen
    岳洁瑜
    余立祥
    吴跃进
    唐灿明
    Plasma Science and Technology, 2008, 10 (05) : 640 - 644
  • [46] Genome-wide identification and expression analysis of the GhIQD gene family in upland cotton(Gossypium hirsutum L.)
    DOU Lingling
    LV Limin
    KANG Yangyang
    TIAN Ruijie
    HUANG Deqing
    LI Jiayin
    LI Siyi
    LIU Fengping
    CAO Lingyan
    JIN Yuhua
    LIU Yang
    LI Huaizhu
    WANG Wenbo
    PANG Chaoyou
    SHANG Haihong
    ZOU Changsong
    SONG Guoli
    XIAO Guanghui
    Journal of Cotton Research, 2021, 4 (01) : 28 - 41
  • [47] Identification and genomic location of a reniform nematode (Rotylenchulus reniformis) resistance locus (Ren ari ) introgressed from Gossypium aridum into upland cotton (G. hirsutum)
    Romano, Gabriela Beatriz
    Sacks, Erik J.
    Stetina, Salliana R.
    Robinson, A. Forest
    Fang, David D.
    Gutierrez, Osman A.
    Scheffler, Jodi A.
    THEORETICAL AND APPLIED GENETICS, 2009, 120 (01) : 139 - 150
  • [48] Genetic mapping and identification of Lg~f loci controlling green fuzz in Upland cotton(Gossypium hirsutum L.)
    Dexin Liu
    Xueying Liu
    Yao Su
    Xiao Zhang
    Kai Guo
    Zhonghua Teng
    Jian Zhang
    Dajun Liu
    Zhengsheng Zhang
    The Crop Journal, 2021, 9 (04) : 777 - 784
  • [49] Genome-wide identification and expression analysis of the GhIQD gene family in upland cotton (Gossypium hirsutum L.)
    Lingling DOU
    Limin LV
    Yangyang KANG
    Ruijie TIAN
    Deqing HUANG
    Jiayin LI
    Siyi LI
    Fengping LIU
    Lingyan CAO
    Yuhua JIN
    Yang LIU
    Huaizhu LI
    Wenbo WANG
    Chaoyou PANG
    Haihong SHANG
    Changsong ZOU
    Guoli SONG
    Guanghui XIAO
    Journal of Cotton Research, 4
  • [50] Genome-wide identification and expression analysis of polyamine oxidase genes in upland cotton (Gossypium hirsutum L.)
    Xin-Qi Cheng
    Xue-Feng Zhu
    Wen-Gang Tian
    Wen-Han Cheng
    Jie Hakim
    Shuang-Xia Sun
    Hua-Guo Jin
    Plant Cell, Tissue and Organ Culture (PCTOC), 2017, 129 : 237 - 249