Isolation and characterization of microsatellite markers in Dysosma versipellis (Berberidaceae), a rare endemic from China

被引:5
|
作者
Guan, Bi-Cai [1 ,2 ]
Qiu, Ying-Xiong [1 ]
Fu, Cheng-Xin [3 ]
机构
[1] Zhejiang Univ, Coll Life Sci, Lab Syst & Evolutionary Bot, Dept Biol, Hangzhou 310058, Peoples R China
[2] Nanchang Univ, Coll Life Sci, Nanchang 330029, Peoples R China
[3] Zhejiang Univ, State Conservat Ctr Gene Resources Endangered Wil, Hangzhou 310058, Peoples R China
基金
中国国家自然科学基金;
关键词
Dysosma versipellis; conservation; microsatellite markers; threatened medicinal plant;
D O I
10.1007/s10592-007-9393-0
中图分类号
X176 [生物多样性保护];
学科分类号
090705 ;
摘要
Dysosma versipellis (Berberidaceae) is an important threatened medicinal plant (TMP) species. Here we isolated nine polymorphic microsatellite loci from D. versipellis using a modified biotin-capture method. Our isolated loci provided SSR markers with polymorphism of 2-7 alleles per locus. The expected and observed heterozygosities ranged from 0.507 to 0.864 and from 0.360 to 0.720, respectively. These markers would be the useful tools for analyzing questions concerning population genetic structure and mating system of D. versipellis.
引用
收藏
页码:783 / 785
页数:3
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