Quantification of the cellular uptake of cell-penetrating peptides by MALDI-TOF mass spectrometry

被引：93

作者：

Burlina, F ^{[1
]}

Sagan, S ^{[1
]}

Bolbach, G ^{[1
]}

Chassaing, G ^{[1
]}

机构：

[1] Univ Paris 06, CNRS, UMR 7613, F-75252 Paris, France

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2005年 / 44卷 / 27期

关键词：

analytical methods; drug delivery; isotopic labeling; mass spectrometry; peptides;

D O I：

10.1002/anie.200500477

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

(Figure Presented) The ins and outs of peptides: The internalization efficiencies of three cell-penetrating peptides (CPPs; penetratin, (Arg) 9, and Tat48-59) have been measured by MALDI-TOF MS (see scheme). The method leads to direct detection of the CPPs and allows unambiguous discrimination between membrane-bound and internalized CPP. Furthermore, the cellular uptake efficiencies of several CPPs can be compared in a single experiment. © 2005 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

收藏

页码：4244 / 4247

页数：4

相关论文

共 22 条

[1]

DEROSSI D, 1994, J BIOL CHEM, V269, P10444

[2] Physico-chemical requirements for cellular uptake of pAntp peptide - Role of lipid-binding affinity [J].

Drin, G ;

Mazel, M ;

Clair, P ;

Mathieu, D ;

Kaczorek, M ;

Temsamani, J .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 2001, 268 (05) :1304-1314

[3] In vitro uptake and stability study of pVEC and its all-D analog [J].

Elmquist, A ;

Langel, Ü .

BIOLOGICAL CHEMISTRY, 2003, 384 (03) :387-393

[4] Potential peptide carriers:: Amphipathic proline-rich peptides derived from the n-terminal domain of γ-zein [J].

Fernández-Carneado, J ;

Kogan, MJ ;

Castel, S ;

Giralt, E .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2004, 43 (14) :1811-1814

[5]

Fernandez-Carneado J., 2004, ANGEW CHEM, V116, P1847

[6] A stepwise dissection of the intracellular fate of cationic cell-penetrating peptides [J].

Fischer, R ;

Köhler, K ;

Fotin-Mleczek, M ;

Brock, R .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2004, 279 (13) :12625-12635

[7] A quantitative validation of fluorophore-labelled cell-permeable peptide conjugates:: fluorophore and cargo dependence of import [J].

Fischer, R ;

Waizenegger, T ;

Köhler, K ;

Brock, R .

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, 2002, 1564 (02) :365-374

[8] Arginine-rich peptides - An abundant source of membrane-permeable peptides having potential as carriers for intracellular protein delivery [J].

Futaki, S ;

Suzuki, T ;

Ohashi, W ;

Yagami, T ;

Tanaka, S ;

Ueda, K ;

Sugiura, Y .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (08) :5836-5840

[9] The use of photolabelled peptides to localize the substance-P-binding site in the human neurokinin-1 tachykinin receptor [J].

Girault, S ;

Sagan, S ;

Bolbach, G ;

Lavielle, S ;

Chassaing, G .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1996, 240 (01) :215-222

[10] Coupling of MALDI-TOF mass analysis to the separation of biotinylated peptides by magnetic streptavidin beads [J].

Girault, S ;

Chassaing, G ;

Blais, JC ;

Brunot, A ;

Bolbach, G .

ANALYTICAL CHEMISTRY, 1996, 68 (13) :2122-2126