Preparation and application of PGMA-DVB microspheres via surface-modification with quaternary and phenylboronic acid moiety

被引：19

作者：

Pang, Long ^{[1
]}

Xue, Tingting ^{[1
]}

Cong, Hailin ^{[1
,2
]}

Shen, Youqing ^{[1
]}

Yu, Bing ^{[1
,2
]}

机构：

[1] Qingdao Univ, Coll Mat Sci & Engn, Coll Chem & Chem Engn, Inst Biomed Mat & Engn, Qingdao 266071, Peoples R China

[2] Qingdao Univ, State Key Lab Biofibers & Ecotext, Qingdao 266071, Peoples R China

来源：

COLLOIDS AND SURFACES B-BIOINTERFACES | 2020年 / 188卷

基金：

中国国家自然科学基金;

关键词：

Glycidyl methacrylate; 4-(bromomethyl) phenylboronic acid; Nucleotides; High performance liquid chromatography; HYDROPHILIC INTERACTION CHROMATOGRAPHY; LIGHT-ASSISTED PREPARATION; CHIRAL STATIONARY-PHASE; ANION-EXCHANGERS; NUCLEOTIDE SUPPLEMENTATION; LIQUID-CHROMATOGRAPHY; ION CHROMATOGRAPHY; TERM INFANTS; PERFORMANCE; SEPARATION;

D O I：

10.1016/j.colsurfb.2020.110807

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Porous cross-linked poly (glycidyl methacrylate-divinylbenzene) (PGMA-DVB) particles (7.42 +/- 0.24 mu m in diameter) were prepared by an improved two-step seed swelling polymerization method. The PGMA-DVB particles were further modified with poly (allylamine hydrochloride) (PAH) and 4-(bromomethyl) phenylboronic acid (BPA), which were used as high performance liquid chromatography (HPLC) filler for nucleotides separation. The liquid chromatographic column packing materials successfully achieved complete separation of nucleotides mixture or deoxynucleotides mixture based on non-polar adsorption, hydrogen bonding interaction and electrostatic adsorption. Reckoning on the chemical structure of BPA, nucleotides and deoxynucleotides with same base group were also separated. The column packing materials were durable after over 100 time running or 7 days. It presents a kind of new notion for the separation of nucleotides by HPLC.

引用

页数：9

共 44 条

[21] Characterization of enhanced-fluidity liquid hydrophilic interaction chromatography for the separation of nucleosides and nucleotides [J].

Philibert, Gwenaelle S. ;

Olesik, Susan V. .

JOURNAL OF CHROMATOGRAPHY A, 2011, 1218 (45) :8222-8230

[22] New developments in the preparation of anion exchange media based on hyperbranched condensation polymers [J].

Pohl, Christopher ;

Saini, Charanjit .

JOURNAL OF CHROMATOGRAPHY A, 2008, 1213 (01) :37-44

[23]

Qi N, 2013, ADV MATER RES-KR, V668, P128, DOI [10.4028/www.scientific.net/AMR.668.128, DOI 10.4028/WWW.SCIENTIFIC.NET/AMR.668.128]

[24] Preparation and evaluation of surface-bonded tricationic ionic liquid silica as stationary phases for high-performance liquid chromatography [J].

Qiao, Lizhen ;

Shi, Xianzhe ;

Lu, Xin ;

Xu, Guowang .

JOURNAL OF CHROMATOGRAPHY A, 2015, 1396 :62-71

[25] Novel polymer-based anion-exchangers with covalently-bonded functional layers of quaternized polyethyleneimine for ion chromatography [J].

Shchukina, O. I. ;

Zatirakha, A. V. ;

Uzhel, A. S. ;

Smolenkov, A. D. ;

Shpigun, O. A. .

ANALYTICA CHIMICA ACTA, 2017, 964 :187-194

[26] Covalent molecular imprinting made easy: a case study of mannose imprinted polymer [J].

Shen, Feng ;

Ren, Xueqin .

RSC ADVANCES, 2014, 4 (25) :13123-13125

[27] Synthesis and morphology of polyethylene chains grafted onto the surface of crosslinked polystyrene microspheres [J].

Shi, Liya ;

Bi, Wuguo ;

Chen, Hui ;

Tang, Tao .

JOURNAL OF POLYMER SCIENCE PART A-POLYMER CHEMISTRY, 2007, 45 (19) :4477-4486

[28] Advanced soap-free emulsion polymerization for highly pure, micron-sized, monodisperse polymer particles [J].

Shibuya, Kazuhiro ;

Nagao, Daisuke ;

Ishii, Haruyuki ;

Konna, Mikio .

POLYMER, 2014, 55 (02) :535-539

[29] Serum lipids in preterm infants fed a formula supplemented with nucleotides [J].

Siahanidou, T ;

Mandyla, H ;

Papassotiriou, I ;

Anagnostakis, D .

JOURNAL OF PEDIATRIC GASTROENTEROLOGY AND NUTRITION, 2004, 38 (01) :56-60

[30]

Siqun Chen, 2013, Materials Science Forum, V745-746, P53, DOI 10.4028/www.scientific.net/MSF.745-746.53

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