Preparation and application of PGMA-DVB microspheres via surface-modification with quaternary and phenylboronic acid moiety

被引：19
作者：
Pang, Long [1 ]
Xue, Tingting [1 ]
Cong, Hailin [1 ,2 ]
Shen, Youqing [1 ]
Yu, Bing [1 ,2 ]
机构：
[1] Qingdao Univ, Coll Mat Sci & Engn, Coll Chem & Chem Engn, Inst Biomed Mat & Engn, Qingdao 266071, Peoples R China
[2] Qingdao Univ, State Key Lab Biofibers & Ecotext, Qingdao 266071, Peoples R China
来源：
COLLOIDS AND SURFACES B-BIOINTERFACES | 2020年 / 188卷
基金：
中国国家自然科学基金;
关键词：
Glycidyl methacrylate; 4-(bromomethyl) phenylboronic acid; Nucleotides; High performance liquid chromatography; HYDROPHILIC INTERACTION CHROMATOGRAPHY; LIGHT-ASSISTED PREPARATION; CHIRAL STATIONARY-PHASE; ANION-EXCHANGERS; NUCLEOTIDE SUPPLEMENTATION; LIQUID-CHROMATOGRAPHY; ION CHROMATOGRAPHY; TERM INFANTS; PERFORMANCE; SEPARATION;
D O I：
10.1016/j.colsurfb.2020.110807
中图分类号：
Q6 [生物物理学];
学科分类号：
071011 ;
摘要：
Porous cross-linked poly (glycidyl methacrylate-divinylbenzene) (PGMA-DVB) particles (7.42 +/- 0.24 mu m in diameter) were prepared by an improved two-step seed swelling polymerization method. The PGMA-DVB particles were further modified with poly (allylamine hydrochloride) (PAH) and 4-(bromomethyl) phenylboronic acid (BPA), which were used as high performance liquid chromatography (HPLC) filler for nucleotides separation. The liquid chromatographic column packing materials successfully achieved complete separation of nucleotides mixture or deoxynucleotides mixture based on non-polar adsorption, hydrogen bonding interaction and electrostatic adsorption. Reckoning on the chemical structure of BPA, nucleotides and deoxynucleotides with same base group were also separated. The column packing materials were durable after over 100 time running or 7 days. It presents a kind of new notion for the separation of nucleotides by HPLC.
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页数：9
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共 44 条
[1]   Preparation of Anion-Exchange Resin Based on Styrene-Divinylbenzene Copolymer Obtained by Suspension Polymerization Method [J].
Abbasian, Mojtaba ;
Ghaderi, Ali ;
Namazi, Hassan ;
Entezami, Ali Akbar .
POLYMER-PLASTICS TECHNOLOGY AND ENGINEERING, 2011, 50 (15) :1606-1612
[2]   Electrostatic repulsion hydrophilic interaction chromatography for isocratic separation of charged solutes and selective isolation of phosphopeptides [J].
Alpert, Andrew J. .
ANALYTICAL CHEMISTRY, 2008, 80 (01) :62-76
[3]   Determination of nucleosides and nucleotides in food samples by using liquid chromatography and capillary electrophoresis [J].
Dominguez-Alvarez, Javier ;
Mateos-Vivas, Maria ;
Rodriguez-Gonzalo, Encarnacion ;
Garcia-Gomez, Diego ;
Bustamante-Rangel, Myriam ;
Delgado Zamarreno, Maria-Milagros ;
Carabias-Martinez, Rita .
TRAC-TRENDS IN ANALYTICAL CHEMISTRY, 2017, 92 :12-31
[4]   Simultaneous quantification of free amino acids and 5′-nucleotides in shiitake mushrooms by stable isotope labeling-LC-MS/MS analysis [J].
Dong, Meng ;
Qin, Lei ;
Xue, Jia ;
Du, Ming ;
Lin, Song-Yi ;
Xu, Xian-Bing ;
Zhu, Bei-Wei .
FOOD CHEMISTRY, 2018, 268 :57-65
[5]   Effect of land use on the composition of bacterial and fungal communities in saline-sodic soils [J].
Feng, Haojie ;
Wang, Shunyi ;
Gao, Zideng ;
Wang, Zhaokun ;
Ren, Xueqin ;
Hu, Shuwen ;
Pan, Hong .
LAND DEGRADATION & DEVELOPMENT, 2019, 30 (15) :1851-1860
[6]   Stationary phases for separation of nucleosides and nucleotides by hydrophilic interaction liquid chromatography [J].
Garcia-Gomez, D. ;
Rodriguez-Gonzalo, E. ;
Carabias-Martinez, R. .
TRAC-TRENDS IN ANALYTICAL CHEMISTRY, 2013, 47 :111-128
[7]   A simple ion chromatography method for inorganic anion analysis in edible seaweeds [J].
Gomez-Ordonez, Eva ;
Alonso, Esther ;
Ruperez, Pilar .
TALANTA, 2010, 82 (04) :1313-1317
[8]   Polystyrene-divinylbenzene-glycidyl methacrylate stationary phase grafted with poly (amidoamine) dendrimers for ion chromatography [J].
Guo, Dandan ;
Lou, Chaoyan ;
Zhang, Peimin ;
Zhang, Jiajie ;
Wang, Nani ;
Wu, Shuchao ;
Zhu, Yan .
JOURNAL OF CHROMATOGRAPHY A, 2016, 1456 :113-122
[9]   Immune response to nucleotide-supplemented infant formulae:: systematic review and meta-analysis [J].
Gutierrez-Castrellon, Pedro ;
Mora-Magana, Ignacio ;
Diaz-Garcia, Luisa ;
Jimenez-Gutierrez, Carlos ;
Ramirez-Mayans, Jaime ;
Solomon-Santibanez, Guillermo A. .
BRITISH JOURNAL OF NUTRITION, 2007, 98 :S64-S67
[10]   Effect of dietary nucleotide supplementation on growth and immune function in term infants: a randomized controlled trial [J].
Hawkes, JS ;
Gibson, RA ;
Roberton, D ;
Makrides, M .
EUROPEAN JOURNAL OF CLINICAL NUTRITION, 2006, 60 (02) :254-264
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