The effect of cyclo-DfKRG peptide immobilization on titanium on the adhesion and differentiation of human osteoprogenitor cells

被引：32
作者：
Pallu, S [1 ]
Bourget, C
Bareille, R
Labrugère, C
Dard, M
Sewing, A
Jonczyk, A
Vernizeau, M
Durrieu, MC
Amédéé-Vilamitjana, J
机构：
[1] Univ Bordeaux 2, INSERM, U577, F-33076 Bordeaux, France
[2] Biomet France, F-26903 Valence, France
[3] CNRS, ICMCB, Ctr Caracterisat Mat Avances, F-33067 Pessac, France
[4] Biomet Biomat, D-64271 Darmstadt, Germany
[5] Merck KgaA Preclin Res, D-64271 Darmstadt, Germany
来源：
BIOMATERIALS | 2005年 / 26卷 / 34期
关键词：
cyclo-DfKRG peptide; osteoblast; differentiation; adhesion;
D O I：
10.1016/j.biomaterials.2005.04.054
中图分类号：
R318 [生物医学工程];
学科分类号：
0831 ;
摘要：
This study takes place in the field of development of a bioactive surface of titanium alloys. In this paper, titanium was functionalized with cyclo-DfKRG peptide by coating or grafting using different anchors (thiol or phosphonate) as spacers between the surface and the peptide. Cell adhesion, and differentiation of human osteoprogenitor (HOP) cells arising from human bone marrow were investigated. Our results seem to demonstrate that cyclo-DfYRG peptide coating with a phosphonate anchor and grafting procedure contributes to higher cell adhesion and a strong ALP and Cbfal mRNA expression, after 10 days of cell seeding. At the contrary, this peptide coated with a thiol anchor stimulates differentiation of HOP within 3 days of culture. (c) 2005 Elsevier Ltd. All rights reserved.
引用
收藏
页码：6932 / 6940
页数：9
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