Neuroprotective Effect of Ultrasound Neuromodulation on Kainic Acid- Induced Epilepsy in Mice

被引：18

作者：

Zou, Junjie ^{[1
,2
,3
,4
,5
]}

Yi, Shasha ^{[1
,6
]}

Niu, Lili ^{[1
,7
,8
]}

Zhou, Hui ^{[1
]}

Lin, Zhengrong ^{[1
,9
]}

Wang, Yibo ^{[1
]}

Huang, Xiaowei ^{[1
]}

Meng, Wen ^{[1
]}

Guo, Yanwu ^{[2
,3
,4
,5
]}

Qi, Lin ^{[10
]}

Meng, Long ^{[1
,7
,8
]}

机构：

[1] Chinese Acad Sci, Shenzhen Inst Adv Technol, Shenzhen 518055, Peoples R China

[2] Southern Med Univ, Natl Key Clin Specialty, Guangzhou 510282, Peoples R China

[3] Southern Med Univ, Engn Technol Res Ctr, Educ Minist China, Guangzhou 510282, Peoples R China

[4] Southern Med Univ, Guangdong Prov Key Lab Brain Funct Repair & Regen, Guangzhou 510282, Peoples R China

[5] Southern Med Univ, Zhujiang Hosp, Dept Neurosurg, Guangzhou 510282, Peoples R China

[6] Kunming Med Univ, Sch Rehabil, Kunming 650000, Yunnan, Peoples R China

[7] Shenzhen Inst Adv Technol, CAS Key Lab Hlth Informat, Shenzhen 518055, Peoples R China

[8] Guangdong Hong Kong Macao Greater Bay Area Ctr Br, Guangzhou 510515, Peoples R China

[9] Univ Chinese Acad Sci, Shenzhen Coll Adv Technol, Shenzhen 518055, Peoples R China

[10] Northeastern Univ, Coll Med & Biol Informat Engn, Shenyang 110016, Peoples R China

来源：

IEEE TRANSACTIONS ON ULTRASONICS FERROELECTRICS AND FREQUENCY CONTROL | 2021年 / 68卷 / 09期

基金：

中国国家自然科学基金;

关键词：

Frequency control; Acoustics; Ultrasonic imaging; Epilepsy; neuroprotection; ultrasound neuromodulation; EXPERIMENTAL-MODEL; DOWN-REGULATION; APOPTOSIS; PHOSPHORYLATION; BCL-2; BAX;

D O I：

10.1109/TUFFC.2021.3079628

中图分类号：

O42 [声学];

学科分类号：

070206 ; 082403 ;

摘要：

Preliminary evidence suggests that low-intensity pulsed ultrasound (LIPUS) has neuroprotective effects on ischemic stroke, depression, and other conditions leading to neuronal cell death (e.g., Parkinson's disease). The purpose of this study was to investigate the neuroprotective effects of LIPUS in epileptic mice. Mice were made epileptic through kainic acid (KA) administration and then stimulated with LIPUS. The neuroprotective effect of ultrasound was evaluated by observing the latency, anxiety-like behavior, and levels of proteins related to inflammation, apoptosis, or signaling pathways. The safety of LIPUS was assessed by hematoxylin and eosin (H&E) and Nissl stainings. LIPUS prolonged the latency (Sham: 6.00 +/- 0.26 days; 1-kHz pulse repetition frequency (PRF): 7.00 +/- 0.31 days), improved the anxiety-like behavior, and inhibited the expression of inflammatory factors and apoptosis-related proteins. In addition, H&E and Nissl staining results confirmed that LIPUS did not damage the brain. These findings suggest that LIPUS has neuroprotective effects in mice with KA-induced epilepsy. LIPUS may offer a new therapeutic approach to epilepsy.

引用

页码：3006 / 3016

页数：11

共 46 条

[1] Astrocyte immune responses in epilepsy [J].

Aronica, Eleonora ;

Ravizza, Teresa ;

Zurolo, Emanuele ;

Vezzani, Annamaria .

GLIA, 2012, 60 (08) :1258-1268

[2] Role of JNK isoforms in the kainic acid experimental model of epilepsy and neurodegeneration [J].

Auladell, Carme ;

de Lemos, Luisa ;

Verdaguer, Ester ;

Ettcheto, Miren ;

Busquets, Oriol ;

Lazarowski, Alberto ;

Beas-Zarate, Carlos ;

Olloquequi, Jordi ;

Folch, Jaume ;

Camins, Antoni .

FRONTIERS IN BIOSCIENCE-LANDMARK, 2017, 22 :795-814

[3] SEROTONERGIC DENERVATION PARTIALLY PROTECTS RAT STRIATUM FROM KAINIC ACID TOXICITY [J].

BERGER, M ;

SPERK, G ;

HORNYKIEWICZ, O .

NATURE, 1982, 299 (5880) :254-256

[4] The macrophage response towards LPS and its control through the p38MAPK-STAT3 axis [J].

Bode, Johannes G. ;

Ehlting, Christian ;

Haeussinger, Dieter .

CELLULAR SIGNALLING, 2012, 24 (06) :1185-1194

[5] Role of the JNK/c-Jun/AP-1 signaling pathway in galectin-1-induced T-cell death [J].

Brandt, B. ;

Abou-Eladab, E. F. ;

Tiedge, M. ;

Walzel, H. .

CELL DEATH & DISEASE, 2010, 1 :e23-e23

[6] Transcranial focused ultrasound pulsation suppresses pentylenetetrazol induced epilepsy in vivo [J].

Chen, Sin-Guang ;

Tsai, Chih-Hung ;

Lin, Chia-Jung ;

Cheng-Chia, Lee ;

Yu, Hsiang-Yu ;

Hsieh, Tsung-Hsun ;

Liu, Hao-Li .

BRAIN STIMULATION, 2020, 13 (01) :35-46

[7] Low-Intensity Pulsed Ultrasound Attenuates LPS-Induced Neuroinflammation and Memory Impairment by Modulation of TLR4/NF-κB Signaling and CREB/BDNF Expression [J].

Chen, Tao-Tao ;

Lan, Tsuo-Hung ;

Yang, Feng-Yi .

CEREBRAL CORTEX, 2019, 29 (04) :1430-1438

[8] Aberrant hippocampal neurogenesis contributes to epilepsy and associated cognitive decline [J].

Cho, Kyung-Ok ;

Lybrand, Zane R. ;

Ito, Naoki ;

Brulet, Rebecca ;

Tafacory, Farrah ;

Zhang, Ling ;

Good, Levi ;

Ure, Kerstin ;

Kernie, Steven G. ;

Birnbaum, Shari G. ;

Scharfman, Helen E. ;

Eisch, Amelia J. ;

Hsieh, Jenny .

NATURE COMMUNICATIONS, 2015, 6

[9] Mechanical bioeffects of ultrasound [J].

Dalecki, D .

ANNUAL REVIEW OF BIOMEDICAL ENGINEERING, 2004, 6 :229-248

[10] Epilepsy [J].

Devinsky, Orrin ;

Vezzani, Annamaria ;

O'Brien, Terence J. ;

Jette, Nathalie ;

Scheffer, Ingrid E. ;

de Curtis, Marco ;

Perucca, Piero .

NATURE REVIEWS DISEASE PRIMERS, 2018, 4

← 1 2 3 4 5 →