Characterization of Wheat (Triticum aestivum L.) Cultivars Grown in Lesotho by Storage Proteins

被引：0

作者：

Morojele, M. E. ^{[2
]}

Labuschagne, M. T. ^{[1
]}

机构：

[1] Univ Orange Free State, Dept Plant Sci, ZA-9300 Bloemfontein, South Africa

[2] Small Grains Inst, ZA-9700 Bethlehem, South Africa

来源：

CEREAL RESEARCH COMMUNICATIONS | 2010年 / 38卷 / 04期

关键词：

bread wheat; gliadin; HMW-GS; LMW-GS; SDS-PAGE; SUBUNITS; GLUTENIN; BREAD;

D O I：

10.1556/CRC.38.2010.4.13

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Thirty bread wheat cultivars grown in Lesotho were analysed with sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). High and low molecular weight glutenin subunits (HMW-GS and LMW-GS) and gliadins were used to determine genetic variability and relationships between the cultivars. The HMW-GS could not distinguish the cultivars, while both LMW-GS and gliadins could. The Glu-1 score showed that all the quality classes from excellent to poor were represented in the material, but 11 cultivars had the highest score of 10, indicating excellent quality. Cluster analysis performed using gliadins in combination with LMW-GS generated dendrograms which segregated cultivars according to genetic distance. However, the genetic distance between the cultivars were so close that it could be concluded that they are from the same gene-pool and have been used several times in regional breeding programmes.

引用

页码：560 / 568

页数：9

共 22 条

[1]

Cornish G. B., 2006, Gliadin and glutenin: the unique balance of wheat quality, P243

[2]

De Villiers O. T., 1993, South African Journal of Plant and Soil, V10, P99

[3] CHARACTERIZATION OF POLYCLONAL AND MONOCLONAL ANTIPEPTIDE ANTIBODIES SPECIFIC FOR SOME LOW M(R) SUBUNITS OF WHEAT GLUTENIN AND THEIR USE IN THE DETECTION OF ALLELIC VARIANTS AT GLU-3 LOCI [J].

DENERYPAPINI, S ;

MOREL, MH ;

HOLDER, F ;

BONICEL, J ;

VANREGENMORTEL, MHV .

JOURNAL OF CEREAL SCIENCE, 1995, 22 (03) :225-235

[4]

Gupta R. B., 1988, Proceedings of the seventh international wheat genetics symposium, held at Cambridge, UK, 13-19 July 1988., P943

[5]

Hintze J.L., 1998, NCSS 2000 STAT SYSTE

[6] The high and low molecular weight glutenin subunits and ω-gliadin composition of bread and durum wheats commonly grown in Portugal [J].

Igrejas, G ;

Guedes-Pinto, H ;

Carnide, V ;

Branlard, G .

PLANT BREEDING, 1999, 118 (04) :297-302

[7]

KONAREV VG, 1979, CEREAL CHEM, V56, P272

[8] Genetic relationships between South African wheat cultivars as measured by gliadin banding patterns [J].

Labuschagne, MT ;

de Swardt, AM ;

Viljoen, CD .

PLANT BREEDING, 2000, 119 (03) :280-282

[9]

MAARTENS H, 1999, THESIS U ORANGE FREE

[10]

MENKOVSKA M, 2002, IDENTIFICATION CHARA

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