Isolation of new splice isoforms, characterization and expression analysis of the human septin SEPT8 (KIAA0202)

被引：24

作者：

Bläser, S

Jersch, K

Hainmann, I

Zieger, W

Wunderle, D

Busse, A

Zieger, B

机构：

[1] Univ Freiburg, Childrens Hosp, Dept Pediat & Adolescent Med, D-79106 Freiburg, Germany

[2] Univ Mannheim, Dept Gynecol & Obstet, D-68147 Mannheim, Germany

来源：

GENE | 2003年 / 312卷

关键词：

gene structure/sequencing; SEPT5 (cell division cycle related-1); alternative splicing; immunological reagents;

D O I：

10.1016/S0378-1119(03)00635-8

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

SEPT8 (KIAA0202) is a member of the highly conserved septin family. Septins are membrane-associated GTPases which are involved in cytokinesis and cellular morphogenesis. Using the yeast two-hybrid system and the glutathione-S-transferase pull-down assay we previously had identified the SEPT8 (KIAA0202) as interaction partner of the human septin SEPT5 (cell division cycle related-1, CDCrel-1). Since the complete cDNA sequence of the human septin SEPT8 (KIAA0202) was not known at that time, we isolated new 5' and 3' cDNA sequence of SEPT8 (KIAA0202) by screening three different cDNA libraries. In addition, we performed the characterization of SEPT8 (KIAA0202) and identified new splice variants of SEPT8 (KIAA0202). The expression pattern of SEPT8 (KIAA0202) and its interaction partner SEPT5 (CDCrel-1) is illustrated. (C) 2003 Elsevier Science B.V. All rights reserved.

引用

页码：313 / 320

页数：8

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