Production of scFv, Fab, and IgG of CR3022 Antibodies Against SARS-CoV-2 Using Silkworm-Baculovirus Expression System

被引：11

作者：

Ebihara, Takeru ^{[1
]}

Masuda, Akitsu ^{[1
]}

Takahashi, Daisuke ^{[2
]}

Hino, Masato ^{[3
]}

Mon, Hiroaki ^{[4
]}

Kakino, Kohei ^{[1
]}

Fujii, Tsuguru ^{[5
]}

Fujita, Ryosuke ^{[3
]}

Ueda, Tadashi ^{[2
]}

Lee, Jae Man ^{[5
]}

Kusakabe, Takahiro ^{[4
]}

机构：

[1] Kyushu Univ, Grad Sch Bioresource & Bioenvironm Sci, Lab Insect Genome Sci, Nishi Ku, Motooka 744, Fukuoka 8190395, Japan

[2] Kyushu Univ, Fac Pharmaceut Sci, Lab Prot Struct Funct & Design, Higashi Ku, Fukuoka 8128582, Japan

[3] Kyushu Univ, Lab Sanit Entomol, Fac Agr, Nishi Ku, Motooka 744, Fukuoka 8190395, Japan

[4] Kyushu Univ, Lab Insect Genome Sci, Fac Agr, Nishi Ku, Motooka 744, Fukuoka 8190395, Japan

[5] Kyushu Univ, Lab Creat Sci Insect Ind, Fac Agr, Nishi Ku, Motooka 744, Fukuoka 8190395, Japan

来源：

MOLECULAR BIOTECHNOLOGY | 2021年 / 63卷 / 12期

关键词：

Silkworm-baculovirus expression vector system; CR3022; antibody; scFv; Fab; IgG; SARS-CoV-2; Spike protein; DEPENDENT CELLULAR CYTOTOXICITY; EFFICIENT PRODUCTION; SARS CORONAVIRUS; SPIKE PROTEIN; BINDING; FRAGMENT;

D O I：

10.1007/s12033-021-00373-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

COVID-19, caused by SARS-CoV-2, is currently spreading around the world and causing many casualties. Antibodies against such emerging infectious diseases are one of the important tools for basic viral research and the development of diagnostic and therapeutic agents. CR3022 is a monoclonal antibody against the receptor binding domain (RBD) of the spike protein (S protein) of SARS-CoV found in SARS patients, but it was also shown to have strong affinity for that of SARS-CoV-2. In this study, we produced large amounts of three formats of CR3022 antibodies (scFv, Fab and IgG) with high purity using a silkworm-baculovirus expression vector system. Furthermore, SPR measurements showed that the affinity of those silkworm-produced IgG antibodies to S protein was almost the same as that produced in mammalian expression system. These results indicate that the silkworm-baculovirus expression system is an excellent expression system for emerging infectious diseases that require urgent demand for diagnostic agents and therapeutic agents.

引用

页码：1223 / 1234

页数：12

共 49 条

[21]

Li T, 2020, CHARACTERIZATION SAR, DOI [10.1101/2020.03.16.994152, DOI 10.1101/2020.03.16.994152, 10.1101/2020.03.16.994152.]

[22] The effect of variable domain orientation and arrangement on the antigen-binding activity of a recombinant human bispecific diabody. [J].