Quaternary ammonium polysaccharides for gene delivery

被引：42

作者：

Yudovin-Farber, I

Yanay, C

Azzam, T

Linial, M

Domb, AJ

机构：

[1] Hebrew Univ Jerusalem, Fac Med, Sch Pharm, Dept Med Chem & Nat Prod, IL-91120 Jerusalem, Israel

[2] Hebrew Univ Jerusalem, Dept Biol Chem Life Sci, IL-91905 Jerusalem, Israel

[3] Hebrew Univ Jerusalem, Alex Grass Ctr Drug Design & Synth, IL-91905 Jerusalem, Israel

来源：

BIOCONJUGATE CHEMISTRY | 2005年 / 16卷 / 05期

关键词：

D O I：

10.1021/bc050066p

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Cationic polysaccharides were synthesized by conjugation of various monoquaternary (MQ) ammonium oligoamines to oxidized dextran by reductive amination and tested for gene transfection. Polycations of dextran grafted with MQ ammonium oligoamines of two to four amino groups were investigated for their ability to cause pCMV-GFP encoding for green fluorescence protein and beta-Gal encoding for beta-galactosidase protein transfection on EPC and HEK-293 cell lines. These polycations were expected to strongly complex DNA due to increased surface cationic charge of the carrier, which may result in a higher transfection yield. However, the transfection yields were much lower compared to the parent vector, dextran-spermine conjugate, which was highly effective both in vitro and in vivo.

引用

页码：1196 / 1203

页数：8

共 24 条

[1] HUMAN GENE-THERAPY [J].