Pseudomonas mangrovi sp. nov., isolated from mangrove soil

A Gram-stain-negative, aerobic, non-motile, short-rod-shaped bacterium, designated as strain TC11(T), was isolated from rhizosphere soil of mangrove forest (Kandellaobovata) in Fugong village, Zhangzhou, Fujian, China. Strain TC11(T)- grew at 1545 degrees C (optimum, 35 degrees C), 0-8 % (w/v) NaCl (optimum, 1 %, w/v) and pH 5.5-9.5 (optimum, pH 7.5). Phylogenetic analyses revealed that strain TC11(T)- belonged to a Glade of the genus Pseudomonas and showed the highest sequence similarity of 98.4 % to Pseudomonas fluvialis ASS-1(T) , followed by Pseudomonas oleovorans subsp. oleovorans DSM 1045(T) (97.9 %), Pseudomonas indoloxydans JCM 14246(T) (97.7 %), Pseudomonas guguanensis JCM 18416(T)(97.6%) and Pseudomonas aIcaIiphita JCM 10630(T) (97.5%) on the basis of their 16S rRNA gene sequences. The DNA G+C content was 64.3 mol%. In silico DNA-DNA hybridization and average nucleotide identity values between strain TC11(T)- and the reference strains were 19-22 % and 7278 %, respectively. Studies based on the three housekeeping genes, rpoB, gyrB and rpoD, further confirmed that strain TC11 is a novel member of the genus Pseudomonas. The major fatty acids of strain TC11(T) were C-16:0, summed feature 8 (C-18:1 omega 6C/(C18:1)omega 7c) and summed feature 3 (C-16:1 omega 7C/(C16:1)omega 6c). The sole isoprenoid quinone was Q-9. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. Based on the phenotypic, chemotaxonomic and phylogenetic properties, strain TC11(T) represents a novel species of the genus Pseudomonas, for which the name Pseudomonas mangrovi sp. nov., is proposed. The type strain is TC11(T) (=KCTC 62159=MCCC 1K03499).