Differentiation of Fusobacterium necrophorum subspecies from bovine pathological lesions by RAPD-PCR

被引:5
作者
Narongwanichgarn, W
Kawaguchi, E
Misawa, N
Goto, Y
Haga, T
Shinjo, T
机构
[1] Miyazaki Univ, Fac Agr, Dept Vet Microbiol, Miyazaki 8892192, Japan
[2] Miyazaki Univ, Fac Agr, Dept Vet Publ Hlth, Miyazaki 8892192, Japan
关键词
API 20A test; cattle-bacteria; fusobacterium necrophorum; RAPD-PCR;
D O I
10.1016/S0378-1135(01)00405-9
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Nineteen strains from bovine abscesses identified as Fusobacterium necrophorum by the VPI method were examined by other methods. The API 20A test kit characterized all 19 strains as F. necrophorum. Seven of the strains had haemagglutinating activity and, were classified as E. necrophorum subspecies necrophorum, and the remaining, 12 nonhaemagglutinating strains, were classified as E necrophorum subspecies funduliforme. We used RAPD-PCR with a 10-mer oligonucleotide primer, W1L-2, to confirm this differentiation of the two subspecies. These results suggest that random amplified polymorphic DNA polymerase chain reaction (RAPD-PCR) with a suitable primer can be used as a new tool for the differentiation of F necrophorum subspecies isolated from bovine pathological lesions. (C) 2001 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:383 / 388
页数:6
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