Reversible binding of ethacrynic acid to human serum albumin: Difference circular dichroism study

被引：0
|
作者：
Bertucci, C [1 ]
Nanni, B [1 ]
Salvadori, P [1 ]
机构：
[1] Univ Pisa, Dipartimento Chim & Chim Ind, CNR, Ctr Studio Macromol Stereordinate Otticamente Att, I-56126 Pisa, Italy
来源：
CHIRALITY | 1999年 / 11卷 / 01期
关键词：
ethacrynic acid; HSA binding; stereoselectivity; circular dichroism; interaction between drugs; cys(34) modified albumin;
D O I：
10.1002/(SICI)1520-636X(1999)11:1<33::AID-CHIR6>3.0.CO;2-U
中图分类号：
R914 [药物化学];
学科分类号：
100701 ;
摘要：
The reversible binding of ethacrynic acid was characterized by a difference circular dichroism method. A 2/1 stoichiometry was determined for the [drug]/[HSA] (human serum albumin) complex. The reversible binding of ethacrynic acid to HSA determines direct competition with ligands that selectivity bind to site II and to the fatty acid site. Furthermore, indirect competition was shown for ligands for site I (anti-cooperative) and to site III (cooperative). Chirality 11:33-38, 1999. (C) 1999 Wiley-Liss, Inc.
引用
收藏
页码：33 / 38
页数：6
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