A spectroscopic study on the interaction between gold nanoparticles and hemoglobin

被引：81

作者：

Garabagiu, Sorina ^{[1
]}

机构：

[1] Natl Inst R&D Isotop & Mol Technol, Cluj Napoca 400293, Romania

来源：

MATERIALS RESEARCH BULLETIN | 2011年 / 46卷 / 12期

关键词：

Nanostructures; Luminescence; Thermodynamic properties; BOVINE SERUM-ALBUMIN; FLUORESCENCE; VECTOR; STEP;

D O I：

10.1016/j.materresbull.2011.08.032

中图分类号：

T [工业技术];

学科分类号：

08 ;

摘要：

The interaction between horse hemoglobin and gold nanoparticles was studied using optical spectroscopy. UV-vis and fluorescence spectra show that a spontaneous binding process occurred between hemoglobin and gold nanoparticles. The Soret band of hemoglobin in the presence of gold nanoparticles does not show significant changes, which proves that the protein retained its biological function. A shift to longer wavelengths appears in the plasmonic band of gold nanoparticles upon the attachment of hemoglobin molecules. Gold nanoparticles quench the fluorescence emission of tryptophan residues in the structure of hemoglobin. The Stern-Volmer quenching constant, the binding constant and the number of binding sites were also calculated. Thermodynamic parameters indicate that the binding was mainly due to hydrophobic interactions. (C) 2011 Elsevier Ltd. All rights reserved.

引用

页码：2474 / 2477

页数：4

共 29 条

[1] Uptake and biological effects of chitosan-capped gold nanoparticles on Chinese Hamster Ovary cells [J].

Boca, Sanda C. ;

Potara, Monica ;

Toderas, Felicia ;

Stephan, Olivier ;

Baldeck, Patrice L. ;

Astilean, Simion .

MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS, 2011, 31 (02) :184-189

[2] An ethylene-glycol decorated ruthenium(II) complex for two-photon photodynamic therapy [J].

Boca, Sanda C. ;

Four, Mickael ;

Bonne, Adeline ;

van der Sanden, Boudewijn ;

Astilean, Simion ;

Baldeck, Patrice L. ;

Lemercier, Gilles .

CHEMICAL COMMUNICATIONS, 2009, (30) :4590-4592

[3] Toxic interaction mechanism between oxytetracycline and bovine hemoglobin [J].

Chi, Zhenxing ;

Liu, Rutao ;

Yang, Bingjun ;

Zhang, Hao .

JOURNAL OF HAZARDOUS MATERIALS, 2010, 180 (1-3) :741-747

[4] Gold nanoparticles are taken up by human cells but do not cause acute cytotoxicity [J].

Connor, EE ;

Mwamuka, J ;

Gole, A ;

Murphy, CJ ;

Wyatt, MD .

SMALL, 2005, 1 (03) :325-327

[5] Novel photonic technique creates micrometer resolution protein arrays and provides a new approach to coupling of genes, peptide hormones and drugs to nanoparticle carriers [J].

Duroux, M. ;

Duroux, L. ;

Neves-Petersen, M. T. ;

Skovsen, E. ;

Petersen, S. B. .

APPLIED SURFACE SCIENCE, 2007, 253 (19) :8125-8129

[6] Covalently linked au nanoparticles to a viral vector: Potential for combined photothermal and gene cancer therapy [J].

Everts, M ;

Saini, V ;

Leddon, JL ;

Kok, RJ ;

Stoff-Khalili, M ;

Preuss, MA ;

Millican, CL ;

Perkins, G ;

Brown, JM ;

Bagaria, H ;

Nikles, DE ;

Johnson, DT ;

Zharov, VP ;

Curiel, DT .

NANO LETTERS, 2006, 6 (04) :587-591

[7] Investigation of the interaction between acridine orange and bovine serum albumin [J].

Feng, XZ ;

Lin, Z ;

Yang, LJ ;

Wang, C ;

Bai, CL .

TALANTA, 1998, 47 (05) :1223-1229

[8] Simple hemoglobin-gold nanoparticles modified electrode for the amperometric detection of acrylamide [J].

Garabagiu, Sorina ;

Mihailescu, Gheorghe .

JOURNAL OF ELECTROANALYTICAL CHEMISTRY, 2011, 659 (02) :196-200

[9] Static and Dynamic Interaction of a Naturally Occurring Photochromic Molecule with Bovine Serum Albumin Studied by UV-Visible Absorption and Fluorescence Spectroscopy [J].

Gentili, Pier Luigi ;

Ortica, Fausto ;

Favaro, Gianna .

JOURNAL OF PHYSICAL CHEMISTRY B, 2008, 112 (51) :16793-16801

[10] Hydrogen bonding to Trp β37 is the first step in a compound pathway for hemoglobin allostery [J].

Goldbeck, RA ;

Esquerra, RM ;

Kliger, DS .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2002, 124 (26) :7646-7647

← 1 2 3 →