Ternary Monolayer Interfaces for Ultrasensitive and Direct Bioelectronic Detection of Nucleic Acids in Complex Matrices

被引：19

作者：

Campuzano, S. ^{[1
,2
]}

Kuralay, F. ^{[1
,3
]}

Wang, J. ^{[1
]}

机构：

[1] Univ Calif San Diego, Dept Nanoengn, San Diego, CA 92903 USA

[2] Univ Complutense Madrid, Dpto Quim Analit, Fac CC Quim, E-28040 Madrid, Spain

[3] Ordu Univ, Dept Chem, TR-52200 Ordu, Turkey

来源：

ELECTROANALYSIS | 2012年 / 24卷 / 03期

基金：

美国国家卫生研究院;

关键词：

Self-assembled monolayers; DNA; Surface chemistry; Ternary monolayers; Nucleic acid hybridization; Biological agents; Dithiols; Biological fluids; SELF-ASSEMBLED MONOLAYERS; PRINTED CARBON ELECTRODES; ELECTROCHEMICAL DNA; GOLD ELECTRODES; ESCHERICHIA-COLI; HYBRIDIZATION DETECTION; IMPEDANCE SPECTROSCOPY; MAGNETIC BEADS; BIOSENSORS; GENE;

D O I：

10.1002/elan.201100452

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Electrochemical nucleic acid biosensors have received considerable attention for the detection of biological agents. Control of the surface chemistry and coverage of the electrode transducer are essential for enhancing the performance of electrochemical DNA biosensors, and particularly for maximizing the hybridization efficiency and minimizing of nonspecific adsorption events. This review highlights recent advances and progress in the development of new surface chemistry approaches based on novel ternary DNA self-assembled monolayer (SAM)-interfaces using dithiols as new co-adsorbents/backfillers. The design and characterization of these powerful ternary SAM interfaces on different gold transducers are described, along with their implications to electrochemical biosensing of bioagents.

引用

页码：483 / 493

页数：11

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