Human ileostomy glycoproteins as a model for small intestinal mucus to investigate adhesion of probiotics

被引：58
作者：
Tuomola, EM [1 ]
Ouwehand, AC [1 ]
Salminen, SJ [1 ]
机构：
[1] Univ Turku, Dept Biochem & Food Chem, FIN-20014 Turku, Finland
来源：
LETTERS IN APPLIED MICROBIOLOGY | 1999年 / 28卷 / 03期
关键词：
D O I：
10.1046/j.1365-2672.1999.00518.x
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Human ileostomy glycoproteins mere used as a model for small intestinal mucus to investigate the adhesion of 12 Lactobacillus strains, one Lactococcus strain and one Propionibacterium strain. Both probiotic and dairy strains were tested. Adhesive and non-adhesive Escherichia coli strains were used as controls. All the strains were also nested for their adhesion to polystyrene. Adhesion to ileostomy glycoproteins and to polystyrene varied significantly among the strains tested. Lactobacillus rhamnosus (human isolate), Lactobacillus GG, Lact. acidophilus 1 and P. freudenreichii adhered to ileostomy glycoproteins. Adhesion was concentration-dependent and the most adhesive strains were able to saturate the substratum. These results indicate that human ileostomy glycoproteins can be used as a model system to select potential probiotic strains to complement the adhesion test with intestinal cell lines.
引用
收藏
页码：159 / 163
页数：5
相关论文
共 21 条
[1]   Recovery of Lactobacillus rhamnosus GG from human colonic biopsies [J].
Alander, M ;
Korpela, R ;
Saxelin, M ;
VilpponenSalmela, T ;
MattilaSandholm, T ;
vonWright, A .
LETTERS IN APPLIED MICROBIOLOGY, 1997, 24 (05) :361-364
[2]   LACTOBACILLUS-ACIDOPHILUS LA-1 BINDS TO CULTURED HUMAN INTESTINAL-CELL LINES AND INHIBITS CELL ATTACHMENT AND CELL INVASION BY ENTEROVIRULENT BACTERIA [J].
BERNET, MF ;
BRASSART, D ;
NEESER, JR ;
SERVIN, AL .
GUT, 1994, 35 (04) :483-489
[3]   ADHESION OF HUMAN BIFIDOBACTERIAL STRAINS TO CULTURED HUMAN INTESTINAL EPITHELIAL-CELLS AND INHIBITION OF ENTEROPATHOGEN-CELL INTERACTIONS [J].
BERNET, MF ;
BRASSART, D ;
NEESER, JR ;
SERVIN, AL .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1993, 59 (12) :4121-4128
[4]  
CARLSTEDTDUKE B, 1989, WENNER-GR C, V52, P109
[5]   PROTEIN-MEDIATED ADHESION OF LACTOBACILLUS-ACIDOPHILUS BG2FO4 ON HUMAN ENTEROCYTE AND MUCUS-SECRETING CELL-LINES IN CULTURE [J].
COCONNIER, MH ;
KLAENHAMMER, TR ;
KERNEIS, S ;
BERNET, MF ;
SERVIN, AL .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1992, 58 (06) :2034-2039
[6]  
COHEN PS, 1995, METHOD ENZYMOL, V253, P309
[7]   ADHESION OF DIFFERENT BIFIDOBACTERIA STRAINS TO HUMAN ENTEROCYTE-LIKE CACO-2 CELLS AND COMPARISON WITH IN-VIVO STUDY [J].
CROCIANI, J ;
GRILL, JP ;
HUPPERT, M ;
BALLONGUE, J .
LETTERS IN APPLIED MICROBIOLOGY, 1995, 21 (03) :146-148
[8]   ATTACHMENT OF LACTOBACILLUS-CASEI STRAIN GG TO HUMAN COLON-CARCINOMA CELL-LINE CACO-2 - COMPARISON WITH OTHER DAIRY STRAINS [J].
ELO, S ;
SAXELIN, M ;
SALMINEN, S .
LETTERS IN APPLIED MICROBIOLOGY, 1991, 13 (03) :154-156
[9]   SURVIVAL OF LACTOBACILLUS SPECIES (STRAIN GG) IN HUMAN GASTROINTESTINAL-TRACT [J].
GOLDIN, BR ;
GORBACH, SL ;
SAXELIN, M ;
BARAKAT, S ;
GUALTIERI, L ;
SALMINEN, S .
DIGESTIVE DISEASES AND SCIENCES, 1992, 37 (01) :121-128
[10]   CLONING AND CHARACTERIZATION OF THE S-FIMBRIAL ADHESIN-II COMPLEX OF AN ESCHERICHIA-COLI O-18-K1 MENINGITIS ISOLATE [J].
HACKER, J ;
KESTLER, H ;
HOSCHUTZKY, H ;
JANN, K ;
LOTTSPEICH, F ;
KORHONEN, TK .
INFECTION AND IMMUNITY, 1993, 61 (02) :544-550
123