Singlet oxygen generation in the reaction centers of Rhodobacter sphaeroides

被引：49

作者：

Uchoa, Adjaci F. ^{[1
]}

Knox, Peter P. ^{[2
]}

Turchielle, Rozane ^{[1
]}

Seifullina, Nurania Kh. ^{[2
]}

Baptista, Mauricio S. ^{[1
]}

机构：

[1] Univ Sao Paulo, Inst Quim, Dept Bioquim, BR-05513970 Sao Paulo, Brazil

[2] Moscow MV Lomonosov State Univ, Dept Biol, Moscow 119992, Russia

来源：

EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS | 2008年 / 37卷 / 06期

基金：

俄罗斯基础研究基金会;

关键词：

photosynthetic bacteria; purple bacteria; photoinhibition; triplets; quantum yield; ROS;

D O I：

10.1007/s00249-008-0287-y

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Singlet oxygen ((1)O(2)) generation in the reaction centers (RCs) of Rhodobacter sphaeroides wild type was characterized by luminescent emission in the near infrared region (time resolved transients and emission spectra) and quantified to have quantum yield of 0.03 +/- 0.005. (1)O(2) emission was measured as a function of temperature, ascorbate, urea and potassium ferricyanide concentrations and as a function of incubation time in H(2)O: D(2)O mixtures. (1)O(2) was shown to be affected by the RC dynamics and to originate from the reaction of molecular oxygen with two sources of triplets: photoactive dimer formed by singlet-triplet mixing and bacteriopheophytin formed by direct photoexcitation and intersystem crossing.

引用

页码：843 / 850

页数：8

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