Investigation of Enantiomeric Separation of Chiral Drugs by CE Using Cu(II)-Clindamycin Complex as a Novel Chiral Selector

被引：9

作者：

Wu, Jianfeng ^{[1
]}

Liu, Peng ^{[1
]}

Wang, Qingwei ^{[1
]}

Chen, Hui ^{[1
]}

Gao, Peng ^{[1
]}

Wang, Li ^{[1
]}

Zhang, Shengyong ^{[1
]}

机构：

[1] Fourth Mil Med Univ, Res Ctr Chirotechnol, Xian 710032, Peoples R China

来源：

CHROMATOGRAPHIA | 2011年 / 74卷 / 11-12期

关键词：

Capillary electrophoresis; Cu(II)-clindamycin complexes; Chiral selector; Enantiomeric separation; EXCHANGE CAPILLARY-ELECTROPHORESIS; MICELLAR ELECTROKINETIC CHROMATOGRAPHY; ACID ENANTIOMERS; AMINO-ACIDS; ENANTIOSEPARATION; DIPEPTIDES; RESOLUTION;

D O I：

10.1007/s10337-011-2138-8

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The enantiomeric separation of several basic drugs was investigated using copper(II)-clindamycin as a new chiral selector. The results show that the chiral selector allows high-resolution separation of some racemic basic drugs, including tropicamide, propranolol, sotalol, bisoprolol, epinephrine, esmolol, atenolol, and metoprolol. The enantioselectivity was influenced by parameters such as the type of metal ion, ratio of clindamycin and Cu(II), pH of the background electrolyte, clindamycin concentration, applied voltage, and capillary temperature. The optimal separation conditions were determined to be 20 mM clindamycin/10 mM Cu(2+), pH 9.06, at 20 kV and 22 A degrees C within 25 min.

引用

页码：789 / 797

页数：9

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