Direct Identification of Staphylococcus aureus and Determination of Methicillin Susceptibility From Positive Blood-Culture Bottles in a Bact/ALERT System Using Binax Now S. aureus and PBP2a Tests

被引：13

作者：

Heraud, Sandrine ^{[1
]}

Freydiere, Anne-Marie ^{[1
,2
,3
,4
]}

Doleans-Jordheim, Anne ^{[1
,5
]}

Bes, Michele ^{[1
,2
,3
]}

Tristan, Anne ^{[1
,2
,3
,4
]}

Vandenesch, Francois ^{[1
,2
,3
,4
]}

Laurent, Frederic ^{[2
,3
,4
,6
]}

Dauwalder, Olivier ^{[1
,2
,3
,4
]}

机构：

[1] Hosp Civils Lyon, Bacteriol Lab, Ctr Biol & Pathol Est, Bron, France

[2] Hosp Civils Lyon, Ctr Natl Reference Staphylocoques, Ctr Biol & Pathol Est, Bron, France

[3] INSERM, U1111, Int Ctr Res Infectiol, F-69008 Lyon, France

[4] Univ Lyon, Fac Med Lyon Est, Lyon, France

[5] Univ Lyon 1, Res Grp Bacterial Opportunist Pathogens & Environ, UMR Ecol Microbienne 5557, CNRS,ENVL, F-69365 Lyon, France

[6] Hosp Civils Lyon, Bacteriol Lab, Ctr Biol & Pathol Nord, Lyon, France

来源：

ANNALS OF LABORATORY MEDICINE | 2015年 / 35卷 / 04期

关键词：

Staphylococcus aureus; Methicillin resistance; Blood culture; Accuracy; Immunochromatographic test; RAPID IDENTIFICATION; ASSAY; RESISTANCE; COST; MECA;

D O I：

10.3343/alm.2015.35.4.454

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

Staphylococcus aureus bacteremia is associated with high mortality and morbidity, requiring prompt and appropriate antimicrobial treatment. Therefore, it is important to detect methicillin-resistant S. aureus (MRSA) rapidly from blood cultures. Two immunochromatographic tests, BinaxNow S. aureus and BinaxNow PBP2a, were directly applied to 79 Bact/Alert bottles that were positive for Gram positive cocci in cluster aggregations. Sensitivity and specificity for the identification of S. aureus and determination of methicillin resistance were 94% and 87%, and 100% and 100%, respectively, with less than 30 min of performance time. These tests are efficient and rapid; these tests are valuable alternatives to more sophisticated and expensive methods used in the diagnosis of MRSA bacteremia.

引用

页码：454 / 457

页数：4

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