Characterization of intracellular and extracellular saxitoxin levels in both field and cultured Alexandrium spp. samples from Sequim Bay, Washington

被引：47

作者：

Lefebvre, Kathi A. ^{[1
]}

Bill, Brian D. ^{[1
]}

Erickson, Aleta ^{[2
]}

Baugh, Keri A. ^{[1
]}

O'Rourke, Lohna ^{[2
]}

Costa, Pedro R. ^{[3
]}

Nance, Shelly ^{[1
]}

Trainer, Vera L. ^{[1
]}

机构：

[1] NOAA Fisheries, NW Fisheries Sci Ctr, Marine Biotoxins Program, Seattle, WA 98122 USA

[2] Jamestown Sklallam Tribe, Sequim, WA 98382 USA

[3] Natl Inst Agron & Fisheries Res, IPIMAR, P-1449006 Lisbon, Portugal

来源：

MARINE DRUGS | 2008年 / 6卷 / 02期

关键词：

PSP; PSTs; harmful algal blooms; saxitoxin; Alexandrium spp; extracellular toxins; intracellular toxins; HAB;

D O I：

10.3390/md20080006

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

Traditionally, harmful algal bloom studies have primarily focused on quantifying toxin levels contained within the phytoplankton cells of interest. In the case of paralytic shellfish poisoning toxins (PSTs), intracellular toxin levels and the effects of dietary consumption of toxic cells by planktivores have been well documented. However, little information is available regarding the levels of extracellular PSTs that may leak or be released into seawater from toxic cells during blooms. In order to fully evaluate the risks of harmful algal bloom toxins in the marine food web, it is necessary to understand all potential routes of exposure. In the present study, extracellular and intracellular PST levels were measured in field seawater samples (collected weekly from June to October 2004-2007) and in Alexandrium spp. culture samples isolated from Sequim Bay, Washington. Measurable levels of intra- and extra-cellular toxins were detected in both field and culture samples via receptor binding assay (RBA) and an enzyme-linked immunosorbent assay (ELISA). Characterization of the PST toxin profile in the Sequim Bay isolates by pre-column oxidation and HPLC-fluorescence detection revealed that gonyautoxin 1 and 4 made up 65 +/- 9.7 % of the total PSTs present. Collectively, these data confirm that extracellular PSTs are present during blooms of Alexandrium spp. in the Sequim Bay region.

引用

页码：103 / 116

页数：14

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