Reverse-genetics studies of lncRNAs-what we have learnt and paths forward

被引：61

作者：

Gao Fan ^{[1
]}

Cai Ye ^{[1
]}

Kapranov, Philipp ^{[1
]}

Xu Dongyang ^{[1
]}

机构：

[1] Huaqiao Univ, Inst Genom, Sch Biomed Sci, 201 Pan Chinese S&T Bldg,668 Jimei Rd, Xiamen 361021, Peoples R China

来源：

GENOME BIOLOGY | 2020年 / 21卷 / 01期

基金：

美国国家科学基金会;

关键词：

LONG NONCODING RNA; EMBRYONIC-DEVELOPMENT; DOSAGE COMPENSATION; ESSENTIAL REGULATOR; MESSENGER-RNA; KNOCKOUT MICE; XIST RNA; JUNK DNA; IN-VIVO; BC1; RNA;

D O I：

10.1186/s13059-020-01994-5

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Long non-coding RNAs (lncRNAs) represent a major fraction of the transcriptome in multicellular organisms. Although a handful of well-studied lncRNAs are broadly recognized as biologically meaningful, the fraction of such transcripts out of the entire collection of lncRNAs remains a subject of vigorous debate. Here we review the evidence for and against biological functionalities of lncRNAs and attempt to arrive at potential modes of lncRNA functionality that would reconcile the contradictory conclusions. Finally, we discuss different strategies of phenotypic analyses that could be used to investigate such modes of lncRNA functionality.

引用

页数：23

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