High density DNA methylation array with single CpG site resolution

被引：1210
作者：
Bibikova, Marina [1 ]
Barnes, Bret [1 ]
Tsan, Chan [1 ]
Ho, Vincent [1 ]
Klotzle, Brandy [1 ]
Le, Jennie M. [1 ]
Delano, David [1 ]
Zhang, Lu [1 ]
Schroth, Gary P. [1 ]
Gunderson, Kevin L. [1 ]
Fan, Jian-Bing [1 ]
Shen, Richard [1 ]
机构：
[1] Illumina Inc, San Diego, CA 92121 USA
来源：
GENOMICS | 2011年 / 98卷 / 04期
关键词：
DNA methylation; Methylation profiling; Genome-wide; Epigenetics; Array; Infinium; GENOME-WIDE IDENTIFICATION; MICROARRAY; PROMOTERS; PATTERNS; DATABASE; ISLANDS; TECHNOLOGY; METHYLOME; ENHANCERS; GENES;
D O I：
10.1016/j.ygeno.2011.07.007
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
We have developed a new generation of genome-wide DNA methylation Bead Chip which allows high-throughput methylation profiling of the human genome. The new high density BeadChip can assay over 480K CpG sites and analyze twelve samples in parallel. The innovative content includes coverage of 99% of RefSeq genes with multiple probes per gene, 96% of CpG islands from the UCSC database, CpG island shores and additional content selected from whole-genome bisulfite sequencing data and input from DNA methylation experts. The well-characterized Infinium (R) Assay is used for analysis of CpG methylation using bisulfite-converted genomic DNA. We applied this technology to analyze DNA methylation in normal and tumor DNA samples and compared results with whole-genome bisulfite sequencing (WGBS) data obtained for the same samples. Highly comparable DNA methylation profiles were generated by the array and sequencing methods (average R-2 of 0.95). The ability to determine genome-wide methylation patterns will rapidly advance methylation research. (C) 2011 Elsevier Inc. All rights reserved.
引用
收藏
页码：288 / 295
页数：8
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