GENETIC DIVERSITY IN BLACKGRAM [VIGNA MUNGO (L.) HEPPER] FOR RANDOMLY AMPLIFIED POLYMORPHIC DNA (RAPD) MARKERS

被引：0
作者：
Ghafoor, Abdul [1 ]
Sultana, Tayyaba [2 ]
Rizvi, Zarrin Fatima [3 ]
机构：
[1] Natl Agr Res Ctr NARC, Inst Agri Biotechnol & Genet Resources IABGR, Islamabad, Pakistan
[2] GC Univ, Faisalabad, Pakistan
[3] Allama Iqbal Open Univ, Dept Agr Sci, Islamabad, Pakistan
来源：
PAKISTAN JOURNAL OF BOTANY | 2012年 / 44卷 / 02期
关键词：
MOLECULAR MARKERS; LINKAGE MAP; SDS-PAGE; QUANTITATIVE TRAITS; GERMPLASM; SEED; GRAM; DIFFERENTIATION; CONSTRUCTION; PROTEIN;
D O I：
暂无
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
Thirty seven pure-lines of blackgram were studied for Randomly Amplified Polymorphic DNA (RAPD) markers to investigate diversity. Among 53 primers 36 produced polymorphic fragments in blackgram, hence could be used for investigating genetic diversity. Ninety four markers were observed from 11 primers and out of these 72% were polymorphic. The genotypes acquired from Korea, Afghanistan and AVRDC grouped together, whereas genotypes from Pakistan and India were scattered. The RAPD markers were found useful for studying genetic diversity but clustering did not exhibited indication for agronomic performance, whereas quantitative traits contributed more towards agronomic performance. Cluster revealed that only a portion of genetic diversity has been exploited for blackgram improvement that should broaden involving diverse parents from various clusters.
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页码：473 / 478
页数：6
相关论文
共 40 条
[1]  
[Anonymous], 1989, Molecular Cloning
[2]  
[Anonymous], 1976, CGIAR PUBLICATION
[3]  
[Anonymous], 1992, PL BRED RE
[4]  
Arulbalachandran D., 2010, Journal of Crop Science and Biotechnology, V13, P1
[5]   Development of a black gram [Vigna mungo (L.) Hepper] linkage map and its comparison with an azuki bean [Vigna angularis (Willd.) Ohwi and Ohashi] linkage map [J].
Chaitieng, B. ;
Kaga, A. ;
Tomooka, N. ;
Isemura, T. ;
Kuroda, Y. ;
Vaughan, D. A. .
THEORETICAL AND APPLIED GENETICS, 2006, 113 (07) :1261-1269
[6]  
CLARK AG, 1993, MOL BIOL EVOL, V10, P1096
[7]   Genetic differentiation of Vigna species by RAPD, URP and SSR markers [J].
Dikshit, H. K. ;
Jhang, T. ;
Singh, N. K. ;
Koundal, K. R. ;
Bansal, K. C. ;
Chandra, N. ;
Tickoo, J. L. ;
Sharma, T. R. .
BIOLOGIA PLANTARUM, 2007, 51 (03) :451-457
[8]   COMPARISON OF RAPD AND RFLP GENETIC-MARKERS IN DETERMINING GENETIC SIMILARITY AMONG BRASSICA-OLERACEA L GENOTYPES [J].
DOSSANTOS, JB ;
NIENHUIS, J ;
SKROCH, P ;
TIVANG, J ;
SLOCUM, MK .
THEORETICAL AND APPLIED GENETICS, 1994, 87 (08) :909-915
[9]   MOLECULAR MARKERS IN PLANT IMPROVEMENT - MANIPULATION OF GENES AFFECTING QUANTITATIVE TRAITS [J].
DUDLEY, JW .
CROP SCIENCE, 1993, 33 (04) :660-668
[10]   The use of RAPD markers for lentil genetic mapping and the evaluation of distorted F-2 Segregation [J].
Eujayl, I ;
Baum, M ;
Erskine, W ;
Pehu, E ;
Muehlbauer, FJ .
EUPHYTICA, 1997, 96 (03) :405-412
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