Expression of multidrug resistance genes in normal and cancer stem cells

被引：118
作者：
Shervington, A. [1 ]
Lu, C. [1 ]
机构：
[1] Univ Cent Lancashire, Fac Sci & Technol, Preston PR1 2HE, Lancs, England
来源：
CANCER INVESTIGATION | 2008年 / 26卷 / 05期
关键词：
cancer stem cells (CSCs); CD133; drug resistance; gliomas; serum free;
D O I：
10.1080/07357900801904140
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
CD133 (stem marker) and drug resistant gene (mdr1 and bcl-2) expression were detected in human glioblastoma and normal brain tissues. Glioma tissues contained higher ratio of CD133+ cells compared to normal tissues (approximate to 10 fold increase). The chemosensitivity assay showed that GOS-3 and NHA CD133+ possess greater resistance towards drugs compared to CD133- fractions. This study revealed for the first time that: a) serum deprivation enriched CD133 expression and demonstrated a direct co-expression between CD133 and drug resistant in GOS-3 cells and b) higher expression of CD133 and drug resistance were found in glioblastoma tissues in comparison to normal brain tissues.
引用
收藏
页码：535 / 542
页数：8
相关论文
共 22 条
[1]   Immunohistochemical detection of nestin in pediatric brain tumors [J].
Almqvist, PM ;
Mah, R ;
Lendahl, U ;
Jacobsson, B ;
Hendson, G .
JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 2002, 50 (02) :147-158
[2]   ABC transporters as phenotypic markers and functional regulators of stem cells [J].
Bunting, KD .
STEM CELLS, 2002, 20 (01) :11-20
[3]  
Cheong HT, 2003, JPN J VET RES, V51, P95
[4]   Tumour stem cells and drug resistance [J].
Dean, M ;
Fojo, T ;
Bates, S .
NATURE REVIEWS CANCER, 2005, 5 (04) :275-284
[5]   Hematopoietic stem cells need two signals to prevent apoptosis; BCL-2 can provide one of these, Kitl/c-Kit signaling the other [J].
Domen, J ;
Weissman, IL .
JOURNAL OF EXPERIMENTAL MEDICINE, 2000, 192 (12) :1707-1718
[6]   Multiple drug resistance in cancer revisited: The cancer stem cell hypothesis [J].
Donnenberg, VS ;
Donnenberg, AD .
JOURNAL OF CLINICAL PHARMACOLOGY, 2005, 45 (08) :872-877
[7]   Stem cells and brain cancer [J].
Galderisi, U ;
Cipollaro, M ;
Giordano, A .
CELL DEATH AND DIFFERENTIATION, 2006, 13 (01) :5-11
[8]   Cell cycle analysis of the CD133+ and CD133- cells isolated from umbilical cord blood [J].
Grskovic, B ;
Ruzicka, K ;
Karimi, A ;
Qujeq, D ;
Müller, MM .
CLINICA CHIMICA ACTA, 2004, 343 (1-2) :173-178
[9]   The role of ABC transporters in clinical practice [J].
Leonard, GD ;
Fojo, T ;
Bates, SE .
ONCOLOGIST, 2003, 8 (05) :411-424
[10]   Analysis of gene expression and chemoresistance of CDI33+ cancer stem cells in glioblastoma [J].
Liu, Gentao ;
Yuan, Xiangpeng ;
Zeng, Zhaohui ;
Tunici, Patrizia ;
Ng, Hiushan ;
Abdulkadir, Iman R. ;
Lu, Lizhi ;
Irvin, Dwain ;
Black, Keith L. ;
Yu, John S. .
MOLECULAR CANCER, 2006, 5 (1)
123