A simple soft lithographic route to fabrication of poly(ethylene glycol) microstructures for protein and cell patterning

被引：242
作者：
Suh, KY
Seong, J
Khademhosseini, A
Laibinis, PE
Langer, R [1 ]
机构：
[1] MIT, Dept Chem Engn, Cambridge, MA 02139 USA
[2] MIT, Div Biol Engn, Cambridge, MA 02139 USA
来源：
BIOMATERIALS | 2004年 / 25卷 / 03期
关键词：
microfabrication; patterning; immobilization; biosensors; cross-linking;
D O I：
10.1016/S0142-9612(03)00543-X
中图分类号：
R318 [生物医学工程];
学科分类号：
0831 ;
摘要：
We present a simple, direct soft lithographic method to fabricate poly(ethylene glycol) (PEG) microstructures for protein and cell patterning. This lithographic method involves a molding process in which a uniform PEG film is molded with a patterned polydimethylsiloxane stamp by means of capillary force. The patterned surfaces created by this method provide excellent resistance towards non-specific protein and cell adsorption. The patterned substrates consist of two regions: the molded PEG surface that acts as a resistant layer and the exposed substrate surface that promotes protein or cell adsorption. A notable finding here is that the substrate surface can be directly exposed during the molding process due to the ability to control the wetting properties of the polymer on the stamp, which is a key factor to patterning proteins and cells. (C) 2003 Elsevier Ltd. All rights reserved.
引用
收藏
页码：557 / 563
页数：7
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