Effects of oral butyrate supplementation on inflammatory potential of circulating peripheral blood mononuclear cells in healthy and obese males

被引：104

作者：

Cleophas, Maartje C. P. ^{[1
,2
]}

Ratter, Jacqueline M. ^{[1
,2
,3
]}

Bekkering, Siroon ^{[1
,2
]}

Quintin, Jessica ^{[5
]}

Schraa, Kiki ^{[1
]}

Stroes, Erik S. ^{[4
]}

Netea, Mihai G. ^{[1
,2
,6
]}

Joosten, Leo A. B. ^{[1
,2
,7
]}

机构：

[1] Radboud Univ Nijmegen, Dept Internal Med, Med Ctr, Nijmegen, Netherlands

[2] Radboud Univ Nijmegen, RIMLS, Med Ctr, Nijmegen, Netherlands

[3] Wageningen Univ, Div Human Nutr, Nutr Metab & Genom Grp, Wageningen, Netherlands

[4] Univ Amsterdam, Acad Med Ctr, Dept Vasc Med, Amsterdam, Netherlands

[5] Inst Pasteur, Dept Mycol, Immunol Fungal Infect Grp, Paris, France

[6] Univ Bonn, Dept Genom & Immunoregulat, Life & Med Sci Inst LIMES, D-53115 Bonn, Germany

[7] Iuliu Hatieganu Univ Med & Pharm, Dept Med Genet, Cluj Napoca, Romania

来源：

SCIENTIFIC REPORTS | 2019年 / 9卷 / 1期

关键词：

CHAIN FATTY-ACIDS; NF-KAPPA-B; SODIUM-BUTYRATE; GUT; INHIBITION; MICROBIOME; RECEPTORS; CYTOKINES; RESPONSES;

D O I：

10.1038/s41598-018-37246-7

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Sodium butyrate is well-known for its immune-modulatory properties. Studies until now only focused on the in vitro effects of butyrate or assessed local effects in the gut upon butyrate administration. In this trial, we studied the systemic anti-inflammatory effects induced by sodium butyrate supplementation in humans. Nine healthy (Lean) and ten obese (metabolic syndrome group, MetSyn) males were given 4 grams sodium butyrate daily for 4 weeks. PBMCs were isolated before and after supplementation for direct stimulation experiments and induction of trained immunity by oxidized low-density lipoprotein (oxLDL), beta-glucan, or Bacillus Calmette-Guerin vaccine (BCG). Butyrate supplementation moderately affected some of the cytokine responses in the MetSyn group. In the direct stimulation setup, effects of butyrate supplementation were limited. Interestingly, butyrate supplementation decreased oxLDL-induced trained immunity in the MetSyn group for LPS-induced IL-6 responses and Pam3CSK4-induced TNF-alpha responses. Induction of trained immunity by beta-glucan was decreased by butyrate in the MetSyn group for Pam3CSK4-induced IL-10 production. In this study, while having only limited effects on the direct stimulation of cytokine production, butyrate supplementation significantly affected trained immunity in monocytes of obese individuals with metabolic complications. Therefore, oral butyrate supplementation may be beneficial in reducing the overall inflammatory status of circulating monocytes in patients with metabolic syndrome.

引用

页数：10

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