Estimation of Cannabis sativa L. Tissue Culture Conditions Essential for Callus Induction and Plant Regeneration

被引：33

作者：

Wielgus, K. ^{[1
,2
]}

Luwanska, A. ^{[2
]}

Lassocinski, W. ^{[2
]}

Kaczmarek, Z. ^{[3
]}

机构：

[1] Inst Nat Fibres, Dept Biotechnol & Mol Biol, PL-60630 Poznan, Poland

[2] Univ Agr, Dept Biochem & Biotechnol, Poznan, Poland

[3] Inst Plant Genet, Lab Biometry, Poznan, Poland

来源：

JOURNAL OF NATURAL FIBERS | 2008年 / 5卷 / 03期

关键词：

Cannabis sativa; callus induction; plant growth regulators; plant regeneration;

D O I：

10.1080/15440470801976045

中图分类号：

TB3 [工程材料学]; TS1 [纺织工业、染整工业];

学科分类号：

0805 ; 080502 ; 0821 ;

摘要：

The effects of different combinations of plant growth regulators on callus induction and plant regeneration were observed in three Polish monoecious hemp cultivars (Bialobrzeskie, Beniko, Silesia). Callus was induced from different explant sources (fragment of cotyledons, stems, roots) on Daria ind+ medium. There was no considerable difference noticed in callus induction between tested cultivars and explants. However, callus obtained from different genotypes had different ability for organogenesis and plant regeneration. Interaction between tested explant and cultivar has significant effect on the efficiency of plant regeneration: the highest regeneration was observed for cotyledon explant (Beniko), the lowest for stem explants (Silesia).

引用

页码：199 / 207

页数：9

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